Studies on the Technique of Tissue Culture and Rapid Propagation of Bulbils From Lilium regale

Bulbils of Lilium regale were used as explants. Adventitious bud induction and the proliferation were studied. The results showed that the regeneration ability of outer layer scales of Lilium regale was better than the middle ones, which was better than the inner ones. The adaxial side of Lilium regale bulbils in contact with medium upward could produce more and stronger adventitious buds than the adaxial side downward. For outer and middle layer scales, the optimum medium for adventitious bud induction was MS +KT 1.0 mg L-1 + NAA 0.2 mg L-1 + CH 500 mg L-1. The adventitious bud induction rate was 100.0% and each explant could produce 12.7 adventitious buds. For the inner ones, the appropriate medium for adventitious bud induction was MS + ZT 1.5 mg L-1 + CH 500 mg L-1 or MS + KT 1.5 mg L-1 + CH 500 mg L-1. The medium MS + KT 0.5 mg L-1 + NAA 0.1 mg L-1 + CH 500 mg L-1 could make proliferation and rooting completed synchronously. The multiplication coefficient was 7.3.