Investigations on Seroprevalence of Bovine Brucellosis in Northeastern, Sudan

A total of 377 serum samples were collected from un-vaccinated cattle brought for slaughter in Northeastern, Sudan to determine the seroprevalence of Bovine Brucellosis (BB) and to evaluate preliminarily the in-house Rose Bengal Plate Test (RBPT). The RBPT and Indirect Enzyme Linked Immunosorbant Assay (I-ELISA) were used to screen all serum samples. The RBPT was carried out in two approaches, the standard RBPT and modified RBPT (mRBPT). The seroprevalence of BB based on I-ELISA and RBPTs were respectively 15.4% (58/377) and 14.3% (54/377). A total of 6.9% (4/58) of true positive was misdiagnosed as negative by both RBPTs whereas 37% (20/54) of strong positive reaction (+3) by mRBPT was agglutinated weakly (+1) by RBPT.