Genetic Diversity of 18 Bramble Cultivars Introduced Abroad and 8 Wild Materials of Rubus coreanus from China Revealed by RAPD

In this study, the genetic diversity and relationship of 18 bramble cultivars and 8 materials from Rubus coreanus were analyzed by RAPD markers. Of the 85 random primers screened, 20 primers gave reproducible and polymorphic bands. Total 251 bands were generated by using 20 RAPD primers, of which 235 (93.6%) were polymorphic. The average number of bands generated per primer was 12.5 with a range from 5-25. The results showed that the genetic distance between Coreanus 3 and 4, both collected in Xichong County, Sichuan Province, was the nearest, while the genetic distance between yellow raspberry Kiwigold and blackberry Olallie was the farthest. The dendrogram of cluster analysis showed that raspberry cultivars and R. coreanus had close genetic relationship, since they clustered into a group. Black raspberry Bristol and blackberry cultivars clustered into another group. The genetic relationships and diversity within the cultivars or materials from R. coreanus was complex, for they didn t cluster together according to their fruit color or original place.