Comparison of Rapid DNA Extraction Techniques for Conventional PCR-RFLP Analysis from Mammalian Whole Blood Cells

Clean, high molecular weight DNA is pre-requisite for DNA markers. The amount and quality of DNA is a crucial point for all further analysis. A unique advantage of these PCR techniques is the rapid DNA analysis of many animal samples using small quantities of DNA. Thus, a simple and rapid DNA extraction method is needed for studies such as genetic analysis that require large populations. Several methods for minimizing the DNA extraction steps have been reported but they require a large amount of animal tissues. In addition, bleeding and management of sampling and storage of the blood sample in freezers is often difficult due to space constraints. To overcome these problems, some techniques developed a DNA extraction method using the milk or hair root or semen. Researchers compared 4 methods of rapid DNA extraction with isolations of mammalian whole blood samples. DNA extraction methods included boiling, salting out, phenol-chloroform and silica gel procedures. Spectrophotometry and gel monitoring evaluated the DNA yield and purity for the 4 methods. The silica gel and phenol-chloroform methods yielded significantly purity and higher concentration of extracted DNA compared with other DNA extraction methods.