Optimization the Production of the Sticky Ends of Human CCRL1 Gene

CCRL1 encoded by gene belongs to the family of G-protein coupled receptors. This protein is a kind of C-C chemokine receptors which has a basic role in evolutionary function of heart identified chemokines. The aim of this study is to improve the creation of sticky ends at CCRL1 gene by T-vector. In this study, CCRL1 gene was amplified by PCR. Restriction sites of BamHI and NotI enzymes were introdused into the 5′end of the forward and reverse primers, respectively. Mentioned enzymes are in the endonuclease family group therefore can not create sticky ends at both sides of CCRL1 gene to overcome this problem amplified fragment by Taq polymerase enzyme was inserted into T-vector. Then, recombinant vector was transformed in E. coli.