Construction of a Vector Containing Coding Sequence of Lipid Transfer Proten-2 (LTP2) Gene from Rice

Lipid Transfer Proteins (LTPs) are group of proteins with average molecular weight in plants that have potential applications especially in drug delivery systems. This research has been designed with the aim to construction of a vector containing coding sequence of Lipid Transfer Proten-2 (LTP2) gene from rice. There is no intron in rice LTP2 gene. In here, pGEX-6P-2 vector was used. First was extracted rice genome. Then, LTP2 gene was amplified with specific primers and PCR technique. In the next stage vector and LTP2 gene was digested with BamHI and XhoI restriction endonuclease enzymes. Then, digested vector and digested LTP2 gene were ligated in appropriate molar ratios.