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PLOS ONE  2012 

A Cytochrome P450 Conserved in Insects Is Involved in Cuticle Formation

DOI: 10.1371/journal.pone.0036544

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Abstract:

The sequencing of numerous insect genomes has revealed dynamic changes in the number and identity of cytochrome P450 genes in different insects. In the evolutionary sense, the rapid birth and death of many P450 genes is observed, with only a small number of P450 genes showing orthology between insects with sequenced genomes. It is likely that these conserved P450s function in conserved pathways. In this study, we demonstrate the P450 gene, Cyp301a1, present in all insect genomes sequenced to date, affects the formation of the adult cuticle in Drosophila melanogaster. A Cyp301a1 piggyBac insertion mutant and RNAi of Cyp301a1 both show a similar cuticle malformation phenotype, which can be reduced by 20-hydroxyecdysone, suggesting that Cyp301a1 is an important gene involved in the formation of the adult cuticle and may be involved in ecdysone regulation in this tissue.

References

[1]  Feyereisen R (2005) Insect Cytochrome P450. In: Gilbert LI, Iatrou K, Gill SS, editors. Comprehensive Molecular Insect Science. Elsevier. pp. 1–77.
[2]  Lewis DF (2004) 57 varieties: the human cytochromes P450. Pharmacogenomics 5: 305–318.
[3]  Nelson DR (1998) Metazoan cytochrome P450 evolution. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 121: 15–22.
[4]  Nelson DR, Schuler MA, Paquette SM, Werck-Reichhart D, Bak S (2004) Comparative genomics of rice and Arabidopsis: Analysis of 727 cytochrome P450 genes and pseudogenes from a monocot and a dicot. Plant Physiol 135: 756–772.
[5]  Werck-Reichhart D, Feyereisen R (2000) Cytochromes P450: a success story. Genome Biol 1, REVIEWS. 3003 p.
[6]  Gilbert LI (2004) Halloween genes encode P450 enzymes that mediate steroid hormone biosynthesis in Drosophila melanogaster. Mol Cell Endocrinol 215: 1–10.
[7]  Stoilov I (2001) Cytochrome P450s: coupling development and environment. Trends Genet 17: 629–632.
[8]  Zhao Y, Hull AK, Gupta NR, Goss KA, Alonso J, et al. (2002) Trp-dependent auxin biosynthesis in Arabidopsis: involvement of cytochrome P450s CYP79B2 and CYP79B3. Genes Dev 16: 3100–3112.
[9]  Thomas JH (2007) Rapid Birth-Death Evolution Specific to Xenobiotic Cytochrome P450 Genes in Vertebrates. PLoS Genet 3: e67.
[10]  Lee SH, Kang JS, Min JS, Yoon KS, Strycharz JP, et al. (2010) Decreased detoxification genes and genome size make the human body louse an efficient model to study xenobiotic metabolism. Insect Mol Biol. 19: 599–615.
[11]  Strode C, Wondji CS, David JP, Hawkes NJ, Lumjuan N, et al. (2008) Genomic analysis of detoxification genes in the mosquito Aedes aegypti. Insect Biochem Mol Biol 38: 113–123.
[12]  Chavez VM, Marques G, Delbecque JP, Kobayashi K, Hollingsworth M, et al. (2000) The Drosophila disembodied gene controls late embryonic morphogenesis and codes for a cytochrome P450 enzyme that regulates embryonic ecdysone levels. Development 127: 4115–4126.
[13]  Namiki T, Niwa R, Sakudoh T, Shirai K, Takeuchi H, et al. (2005) Cytochrome P450 CYP307A1/Spook: a regulator for ecdysone synthesis in insects. Biochem Biophys Res Commun 337: 367–374.
[14]  Ono H, Rewitz KF, Shinoda T, Itoyama K, Petryk A, et al. (2006) Spook and Spookier code for stage-specific components of the ecdysone biosynthetic pathway in Diptera. Dev Biol 298: 555–570.
[15]  Petryk A, Warren JT, Marques G, Jarcho MP, Gilbert LI, et al. (2003) Shade is the Drosophila P450 enzyme that mediates the hydroxylation of ecdysone to the steroid insect molting hormone 20-hydroxyecdysone. Proc Natl Acad Sci U S A 100: 13773–13778.
[16]  Rewitz KF, Gilbert LI (2008) Daphnia Halloween genes that encode cytochrome P450s mediating the synthesis of the arthropod molting hormone: evolutionary implications. BMC Evol Biol 8: 60.
[17]  Warren JT, Petryk A, Marques G, Jarcho M, Parvy JP, et al. (2002) Molecular and biochemical characterization of two P450 enzymes in the ecdysteroidogenic pathway of Drosophila melanogaster. Proc Natl Acad Sci U S A 99: 11043–11048.
[18]  Warren JT, Petryk A, Marques G, Parvy JP, Shinoda T, et al. (2004) phantom encodes the 25-hydroxylase of Drosophila melanogaster and Bombyx mori: a P450 enzyme critical in ecdysone biosynthesis. Insect Biochem Mol Biol 34: 991–1010.
[19]  Guittard E, Blais C, Maria A, Parvy JP, Parishna S, et al. (2011) CYP18A1, a key enzyme of Drosophila steroid hormone inactivation, is essential for metamorphosis. Dev Biol 349: 35–45.
[20]  Gutierrez ED, Wiggins D, Fielding B, Gould AP (2007) Specialized hepatocyte-like cells regulate Drosophila lipid metabolism. Nature 445: 275–280.
[21]  Maibeche-Coisne ML, Monti-Dedieu S, Aragon S, Dayphin-Villemant C (2000) A new cytochrome P450 from Drosophila melanogaster, CYP4G15, expressed in the nervous system. Biochem Biophys Res Commun 273: 1132–1137.
[22]  Chung H, Sztal T, Pasricha S, Sridhar M, Batterham P, et al. (2009) Characterization of Drosophila melanogaster cytochrome P450 genes. Proc Natl Acad Sci U S A 106: 5731–5736.
[23]  Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, et al. (2000) Artemis: sequence visualization and annotation. Bioinformatics 16: 944–945.
[24]  Thompso , JD , Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882.
[25]  Tamura K, Peterson D, Peterson N, Stecher G, Nei M, et al. (2011) MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance and Maximum Parsimony Methods. Mol Biol Evol 28: 2731–2739.
[26]  Chung H, Bogwitz MR, McCart C, Andrianopoulos A, ffrench-Constant RH, et al. (2007) Cis-regulatory elements in the Accord retrotransposon result in tissue-specific expression of the Drosophila melanogaster insecticide resistance gene Cyp6g1. Genetics 175: 1071–1077.
[27]  Sztal T, Chung H, Gramzow L, Daborn PJ, Batterham P, et al. (2007) Two independent duplications forming the Cyp307a genes in Drosophila. Insect Biochem Mol Biol 37: 1044–53.
[28]  Lee YS, Carthew RW (2003) Making a better RNAi vector for Drosophila: use of intron spacers. Methods 30: 322–329.
[29]  Sekyrova P, Bohmann D, Jindra M, Uhlirova M (2010) Interaction between Drosophila bZIP proteins Atf3 and Jun prevent replacement of epithelial cells during metamorphosis. Development 137: 141–150.
[30]  Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, et al. (2007) Evolution of genes and genomes on the Drosophila phylogeny. Nature 450: 203–218.
[31]  The International Aphid Genomics Consortium IAG (2010) Genome sequence of the pea aphid Acyrthosiphon pisum. PLoS biology 8: e1000313.
[32]  Feyereisen R (2006) Evolution of insect P450. Biochemical Soc Trans 34: 1252–1255.
[33]  Kirkness EF, Haas BJ, Sun W, Braig HR, Perotti MA, et al. (2010) Genome sequences of the human body louse and its primary endosymbiont provide insights into the permanent parasitic lifestyle. Proc Natl Acad Sci U S A 107: 12168–12173.
[34]  Chintapalli VR, Wang J, Dow JA (2007) Using FlyAtlas to identify better Drosophila melanogaster models of human disease. Nat Genet 39: 715–720.
[35]  Thibault ST, Singer MA, Miyazaki WY, Milash B, Dompe NA, et al. (2004) A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. Nat Genet 36: 283–287.
[36]  Horn C, Offen N, Nystedt S, Hacker U, Wimmer EA (2003) piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics. Genetics 163: 647–661.
[37]  Lee T, Luo L (1999) Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron 22: 451–461.
[38]  Riddiford LM (1994) Advances of Insect Physiology. London: Academic Press. 213 p.
[39]  Yao TP, Segrave WA, Oro AE, McKeown M, Evans RM (1992) Drosophila ultraspiracle modulates ecdysone receptor function via heterodimer formation. Cell 71: 63–72.
[40]  Milner MJ (1997) The eversion and differentiation of Drosophila melanogaster leg and wing imaginal discs cultured in vitro with an optimal concentration of beta-ecdysone. J. Embryol. Exp. Morphol 37: 105–117.
[41]  Ninov N, Chiarelli DA, Martin-Blanco E (2007) Extrinsic and intrinsic mechanisms directing epithelial cell sheet replacement during Drosophila metamorphosis. Development 134: 367–379.
[42]  Li X, Schuler MA, Berenbaum MR (2007) Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics. Annu Rev Entomol 52: 231–253.
[43]  Rewitz KF, Rybczynski R, Warren JT, Gilbert LI (2006) Developmental expression of Manduca shade, the P450 mediating the final step in molting hormone synthesis. Mol Cell Endocrinol 247: 166–174.
[44]  Rewitz KF, Rybczynski R, Warren JT, Gilbert LI (2006) Identification, characterization and developmental expression of Halloween genes encoding P450 enzymes mediating ecdysone biosynthesis in the tobacco hornworm, Manduca sexta. Insect Biochem Mol Biol 36: 188–199.
[45]  Rewitz KF, Rybczynski R, Warren JT, Gilbert LI (2006) The Halloween genes code for cytochrome P450 enzymes mediating synthesis of the insect moulting hormone. Biochemical Soc Trans 34: 1256–1260.
[46]  Li L, Chang Z, Pan Z, Fu ZQ, Wang X (2008) Modes of heme binding and substrate access for cytochrome P450 CYP74A revealed by crystal structures of allene oxide synthase. Proc Natl Acad Sci U S A 105: 13883–13888.
[47]  Madhaven MM, Madhaven K (1980) Morphogenesis of the epidermis of adult abdomen of Drosophila. J. Embryol. Exp. Morphol 60: 1–31.
[48]  Roseland CR, Schneiderman HA (1979) Regulation and metamorphosis of the abdominal histoblasts of Drosphila melanogaster. Dev Genes Evol 186: 235–265.
[49]  Bischoff M, Cseresnyes Z (2009) Cell rearrangements, cell divisions and cell death in a migrating epithelial sheet in the abdomen of Drosophila. Development 136: 2403–2411.
[50]  Yao Q, Zhang D, Tang B, Chen J, Chen J, et al. (2010) Identification of 20-hydroxyecdysone late-response genes in the chitin biosynthesis pathway. PLoS One 18: e14058.
[51]  Lafont R, Dauphin-Villemant C, Warren JT, Rees H (2005) Ecdysteroid Chemistry and Biochemistry. In: Gilbert L, Gill SS, editors. Comprehensive Molecular Insect Science. Oxford: Elseveir. pp. 125–195.

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