Lipolytic Activity of some Strains of Klebsiella, Pseudomonas and Staphylococcus Spp. from Restaurant Wastewater and Receiving Stream

The tendency of some lipolytic bacteria isolated from restaurant wastewater and the receiving stream to biodegrade/utilizes fresh palm oil was investigated. Thirty two (32) lipolytic bacteria isolates were identified and grouped into six genera namely; Enterococcus, Escherichia, Klebsiella, Pseudomonas, Serratia and Staphylococcus. The weight of these isolates after 24h of inoculation ranged between 0.33 and 0.60mg, whereas the final weight on the 12th day of incubation was between 0.25mg and 0.51mg. Generally, the optimum growth in all the thirty two (32) lipolytic isolates in the fresh oil was observed between the fifth and seventh days. The growth rate per day were 0.02mg in Pseudomonas sp. (n); 0.03mg in Klebsiella sp. (m); 0.04mg in Pseudomonas sp. (j); 0.04mg in Staphylococcus sp. (r); and 0.05mg in Staphylococcus sp. (p).The appreciable enzymatic activity of these microbes ranged; Lipase (0.036 - 0.073 mM/min/ml) with Pseudomonas sp. (n) shown a highest lipase activity (0.073 mM/min/ml) after 12h, protease (50 - 117mM/min/ml) and amylase (7.7 - 117mM/min/ml). It appears that bacteria associated with dietary oil-rich wastewater are the novel source of environmental enzymes for possible commercial applications and may play an important role in enzyme-catalyzed organic matter cycling in domestic environments.