Effect of pulsed electromagnetic field on inflammatory pathway markers in RAW 264.7 murine macrophages

Effect of pulsed electromagnetic field on inflammatory pathway markers in RAW 264.7 murine macrophages Original Research (445) Total Article Views Authors: Ross CL, Harrison BS Published Date March 2013 Volume 2013:6 Pages 45 - 51 DOI: http://dx.doi.org/10.2147/JIR.S40269 Received: 13 November 2012 Accepted: 11 December 2012 Published: 12 March 2013 Christina L Ross,1,2 Benjamin S Harrison2 1Akamai University, Department of Energy Medicine, Hilo, HI, USA; 2Wake Forest Institute for Regenerative Medicine, Wake Forest Baptist Health, Winston-Salem, NC, USA Abstract: In the treatment of bacterial infections, antibiotics have proven to be very effective, but the way in which antibiotics are dosed can create a lag time between the administration of the drug and its absorption at the site of insult. The time it takes an antibiotic to reach therapeutic levels can often be significantly increased if the vascular system is compromized. Bacteria can multiply pending the delivery of the drug, therefore, developing treatments that can inhibit the inflammatory response while waiting for antibiotics to take effect could help prevent medical conditions such as septic shock. The aim of this study was to examine the effect of a pulsed electromagnetic field on the production of inflammatory markers tumor necrosis factor (TNF), transcription factor nuclear factor kappa B (NFkB), and the expression of the A20 (tumor necrosis factor-alpha-induced protein 3), in an inflamed-cell model. Lipopolysaccharide-challenged cells were exposed to a pulsed electromagnetic field at various frequencies in order to determine which, if any, frequency would affect the TNF-NFkB-A20 inflammatory response pathway. Our study revealed that cells continuously exposed to a pulsed electromagnetic field at 5 Hz demonstrated significant changes in the downregulation of TNF-α and NFkB and also showed a trend in the down regulation of A20, as compared with controls. This treatment could be beneficial in modulating the immune response, in the presence of infection. Keyword: TNFAIP3, pulsed electromagnetic field, macrophages, TNF, NFkB Post to: Cannotea Citeulike Del.icio.us Facebook LinkedIn Twitter Readers of this article also read: The effect of a peptide-containing synthetic lung surfactant on gas exchange and lung mechanics in a rabbit model of surfactant depletion In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia Comparative diagnostic study of biomarkers using FibroMax and pathology for prediction of liver steatosis in patients with chronic hepatitis C virus infection: an Egyptian study Differential characteristics of incobotulinumtoxinA and its use in the management of glabellar frown lines Tumor-targeted liposomal drug delivery mediated by a diseleno bond-stabilized cyclic peptide Safety comparison of additives in antiglaucoma prostaglandin (PG) analog ophthalmic formulations Sevoflurane attenuates pulmonary inflammation and ventilator-