Subacute toxicity of cadmium on hepatocytes and nephrocytes in the rat could be considered as a green biosynthesis of nanoparticles

bacute toxicity of cadmium on hepatocytes and nephrocytes in the rat could be considered as a green biosynthesis of nanoparticles Original Research (325) Total Article Views Authors: Trabelsi H, Azzouz I, Sakly M, Abdelmelek H Published Date March 2013 Volume 2013:8 Pages 1121 - 1128 DOI: http://dx.doi.org/10.2147/IJN.S39426 Received: 22 October 2012 Accepted: 22 December 2012 Published: 14 March 2013 Hamdi Trabelsi, Inès Azzouz, Mohsen Sakly, Hafedh Abdelmelek Laboratory of Physiologie Intégrée, Faculty of Sciences of Bizerte, Carthage University, Tunisia Abstract: The purpose was to study the toxicity of cadmium (Cd) and to explore its potential to generate nanoparticles during detoxification. In order to demonstrate this, in vivo fluorescence imaging, X-ray diffraction, and flow cytometry were performed. The in vivo imaging showed a fluorescence signal after Cd treatment (CdCl2, 1.50 mg/Kg, intraperitoneally). By contrast, the control-rat fluorescence was negative. The fluorescence was divided into three colors, red, yellow, and green, and probably indicates the presence of quantum dots. X-ray diffraction results revealed the presence of Cd sulfide (CdS) and/or Cd selenide (CdSe) nanoparticles following Cd injection in the liver (6.52 nm) and kidneys (56.30 nm). Interestingly, flow cytometry revealed a heterogeneous size distribution and a homogeneous granularity of synthesized nanoparticles. Using the green fluorescence channel and the red fluorescence channel, a narrow green emission spectrum and a broad red emission spectrum were detected, respectively, by cytometric analysis.