Electrospun PGA/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering

trospun PGA/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering Original Research (4950) Total Article Views Authors: Hajiali H, Shahgasempour S, Naimi-Jamal MR, Peirovi H Published Date September 2011 Volume 2011:6 Pages 2133 - 2141 DOI: http://dx.doi.org/10.2147/IJN.S24312 Hadi Hajiali1, Shapour Shahgasempour1, M Reza Naimi-Jamal2, Habibullah Peirovi1 1Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences; 2Department of Chemistry, Iran University of Science and Technology, Tehran, Iran Background and methods: In this study, gelatin was blended with polyglycolic acid (PGA) at different ratios (0, 10, 30, and 50 wt%) and electrospun. The morphology and structure of the scaffolds were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and differential scanning calorimetry. The mechanical properties were also measured by the tensile test. Furthermore, for biocompatibility assessment, human umbilical vein endothelial cells and human umbilical artery smooth muscle cells were cultured on these scaffolds, and cell attachment and viability were evaluated. Results: PGA with 10 wt% gelatin enhanced the endothelial cells whilst PGA with 30 wt% gelatin increased smooth muscle cell adhesion, penetration, and viability compared with the other scaffold blends. Additionally, with the increase in gelatin content, the mechanical properties of the scaffolds were improved due to interaction between PGA and gelatin, as revealed by Fourier transform infrared spectroscopy and differential scanning calorimetry. Conclusion: Incorporation of gelatin improves the biological and mechanical properties of PGA, making promising scaffolds for vascular tissue engineering.