Magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation

gnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation Original Research (2865) Total Article Views Authors: Zeng XB, Hu H, Xie LQ, Lan F, Jiang W, Wu Y, Gu ZW Published Date July 2012 Volume 2012:7 Pages 3365 - 3378 DOI: http://dx.doi.org/10.2147/IJN.S32264 Received: 27 March 2012 Accepted: 28 April 2012 Published: 04 July 2012 Xiao Bo Zeng, Hao Hu, Li Qin Xie, Fang Lan, Wen Jiang, Yao Wu, Zhong Wei Gu National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, People's Republic of China Introduction: In recent years, interest in magnetic biomimetic scaffolds for tissue engineering has increased considerably. A type of magnetic scaffold composed of magnetic nanoparticles (MNPs) and hydroxyapatite (HA) for bone repair has been developed by our research group. Aim and methods: In this study, to investigate the influence of the MNP content (in the scaffolds) on the cell behaviors and the interactions between the magnetic scaffold and the exterior magnetic field, a series of MNP-HA magnetic scaffolds with different MNP contents (from 0.2% to 2%) were fabricated by immersing HA scaffold into MNP colloid. ROS 17/2.8 and MC3T3-E1 cells were cultured on the scaffolds in vitro, with and without an exterior magnetic field, respectively. The cell adhesion, proliferation and differentiation were evaluated via scanning electron microscopy; confocal laser scanning microscopy; and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline phosphatase, and bone gla protein activity tests. Results: The results demonstrated the positive influence of the magnetic scaffolds on cell adhesion, proliferation, and differentiation. Further, a higher amount of MNPs on the magnetic scaffolds led to more significant stimulation. Conclusion: The magnetic scaffold can respond to the exterior magnetic field and engender some synergistic effect to intensify the stimulating effect of a magnetic field to the proliferation and differentiation of cells.