Evaluation of baicalin in Scutellaria baicalensis Georgi using HPLC method

High-performance liquid chromatography (HPLC) has been evaluated for the determination of components in Scutellaria baicalensis georgi, primarily by the separation of baicalin. The samples were extracted at reflux by methanol, and the content of chlorogenic acid was analyzed by HPLC. A Diamonsil C18 column (4.6 mm x 150 mm, 5 μm) was used as the analytical column. The mobile phase was methanol, water and phosphoric acid (47:53:0.2). The detection wavelength was 280 nm to determine the content of baicalin. The baicalin showed linearity over the range of 0.12-1.2 μg. Its average recovery was 98.6% and RSD was 0.78%. KEY WORDS: High performance liquid chromatography, Scutellaria baicalensis georgi, Baicalin Bull. Chem. Soc. Ethiop. 2010, 24(1), 115-119.