Micropropagation of Rosa rugosa Through Axillary Shoot Proliferation

A successful in vitro propagation system has been developed for the valuable ornamental and medicinal plant rugosa rose (Rosa rugosa Thunb.) by in vitro culture of nodal segments from mature plants. MS media supplemented with BA 2.2 μM, NAA 0.054 μM, GA3 2.0 μM and 3% sucrose significantly improved bud break and growth directly from nodal segment explants. High proliferation and average height of shoots were achieved using MS media supplemented with 3% sucrose or glucose adjusted for each cultivar. All the cultivars studied proliferated on MS medium supplemented with BA (2.2 μM), NAA (0.054 μM) and GA3 (0.4 μM). Rooting of shoots was achieved on half-strength MS medium with IBA (2.5-5.0 μM) combined with glucose or sucrose depending on the cultivar. Plantlets were successfully transferred to pots for acclimatization after they had grown more than 3 roots and average root length exceeded 10 mm.