Optimization of Conditions for Charantin Extraction in PEG/Salt Aqueous Two-Phase Systems Using Response Surface Methodology

Momordica charantia, commonly known as karela or bitter melon, is used as a vegetable in Chinese, Indian subcontinent. However, in Chinese traditional medicine, the plant is usually used as an antidiabetic agent. One of the active components is charantin. Aqueous two-phase systems (ATPS) were used for the purifying saponin because of their low cost. ATPS can reduce amount of organic solvent and the danger of explosion necessitates special equipment leading to increased costs. The levels of blood glucose decreased after administration of charantin purified from Momordica charantia with (ATPS (p<0.05).