Investigation of HPV infection in men remains important due to its association with genital warts and anorectal cancer, as well as to the role men play in HPV transmission to their female sexual partners. Asymptomatic men (n = 43), whose sexual partners had presented cervical HPV infection, were enrolled in this study. Among the 43 men, 23 had their female partner included and tested for HPV-DNA, totaling 23 couples. HPV-DNA was detected by PCR. Type specific PCR to detect HPV 16, 18, 31, 33, 45 and 6/11 was performed. At least one type of HPV was detected in 86.0% (37/43) of the male patients and more than one HPV type was identified in 39.5% (17/43) of the samples, including high and low risk HPV. HPV-16 proved to be the most prevalent viral type in both male and female samples. Concordance of at least one viral type was observed in 56.5% (13/23) of the couples. Among couples that have shown concordance of viral types, 84.6% (11/13) of the men had the same high risk viral type presented by the female sexual partner. These data suggest that HPV infected men is an important reservoir, contributing to a higher transmission to women and maintenance of infection, and consequently, a higher risk of developing cervical cancer. HPV vaccination in men will protect not only them but will also have implications for their sexual partners.
References
[1]
Chaturvedi AK, Engels EA, Pfeiffer RM, Hernandez BY, Xiao W, et al. (2011) Human papillomavirus and rising oropharyngeal cancer incidence in the United States. J Clin Oncol. 29: 4294–4301.
[2]
Jin F, Stein AN, Conway EL, Regan DG, Law M, et al. (2011) Trends in anal cancer in Australia, 1982–2005. Vaccine. 29: 2322–2327.
[3]
Tota JE, Chevarie-Davis M, Richardson LA, Devries M, Franco EL (2011) Epidemiology and burden of HPV infection and related diseases: implications for prevention strategies. Prev Med. 53: S12–21.
[4]
Burchell AN, Tellier PP, Hanley J, Coutlée F, Franco EL (2010) Human Papillomavirus Infections Among Couples in New Sexual Relationships. Epidemiology 21: 31–37.
[5]
Dunne EF, Nielson CM, Stone KM, Markowitz LE, Giuliano AR (2006) Prevalence of HPV infection among men: A systematic review of the literature. J Infect Dis 194: 1044–1057.
[6]
Hernandez BY, Wilkens LR, Zhu X, Thompson P, Mcduffie K, et al. (2008) Transmission of human papillomavirus in heterosexual couples. Emerg Infect Dis 14: 888–894.
[7]
Partridge JM & Koutsky LA. 2006. Genital human papillomavirus infection in men. Lancet 6: 21–31.
[8]
Daling JR, Madeleine MM, Johnson LG, Schwartz SM, Shera KA, et al. (2005) Penile cancer: importance of circumcision, human papillomavirus and smoking in in situ and invasive disease. Int J Cancer 116: 606–616.
[9]
Franceschi S, Castellsague X, Dal Maso L (2002) Prevalence and determinants of human papillomavirus genital infection in men. Br J Cancer 86: 705–711.
[10]
Giuliano AR, Tortolero-Luna G, Ferrer E, Burchell AN, Sanjose S, et al. (2008) Epidemiology of Human Papillomavirus Infection in Men, Cancers other than Cervical and Benign Conditions. Vaccine 26: K17–K28.
[11]
Bleeker MCG, Hogewoning CJA, Voorhorst FJ, Van Den Brule AJC, Berkhof J, et al. (2005) HPV – associated flat penile lesions in men of a non-STD hospital population: less frequent and smaller in size than in male sexual partners of women with CIN. Int J Cancer 113: 36–41.
[12]
Nicolau SM, Camargo CGC, Stávale JN, Castelo A, D？res GB, et al. (2005) Human papillomavirus DNA detection in male sexual partners of women with genital human papillomavirus infection. Urology 65: 251–255.
[13]
Rombaldi RL, Serafini EP, Villa LL, Vanni AC, Baréa F, et al. (2006) Infection with human papillomaviruses of sexual partners of women having cervical intraepithelial neoplasia. Braz J Med Biol Res 39: 177–187.
[14]
Rosenblatt C, Lucon AM, Pereyra EAG, Pinotti JA, Arap S, et al. (2004) HPV prevalence among partners of women with cervical intraepithelial neoplasia. Int J Gynecol Obstet 84: 156–161.
[15]
Bleeker MCG, Hogewoning CJA, Berkhof J, Voorhorst FJ, Hesselink AT, et al. (2005a) HPV type concordance in sexual couples determines the effect of condoms on regression of flat penile lesions. Br J Cancer 92: 1388–1392.
[16]
Mu？oz N & Bosch FX (1996) The causal link between HPV and cervical cancer and its implications for prevention of cervical cancer. Bull Pan Am Health Organ 30: 362–377.
[17]
Teixeira JC, Derchain SFM, Teixeira LC, Santos CC, Panetta K, et al. (2002) Avalia？？o do parceiro sexual e risco de recidivas em mulheres tratadas por les？es genitais induzidas pelo papilomavírus humano (HPV). Rev Bras Ginecol Obstet 24: 315–320.
[18]
De Lima Rocha MG, Faria FL, Souza MCM, Vago AR, Fernandes APSM, et al. (2008) Detection of Human Papillomavirus Infection in Penile Samples Through Liquid-based Cytology and Polymerase Chain Reaction. Cancer 114: 489–493.
[19]
Saiki RK, Gelfand DH, Stoffel S, Sharf SJ, Higuchi R, et al. (1988) Primer-directed enzimatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491.
[20]
De Roda Husman AM, Walboomers JM, Van den Brule AJ, Meijer CJ, Snijders PJ (1995) The use of general primers GP5 and GP6 elongated at their 3′ ends with adjacent highly conserved sequences improves human papillomavirus detection by PCR. J Gen Virol 76: 1057–1062.
[21]
HPV Study group in men from Brazil, USA and Mexico (2008) Human Papillomavirus infection in men residing in Brasil, Mexico, and the USA. Salud Publica Mex 50: 408–418.
[22]
Depuydt CE, Vereecken AJ, Salembier GM, Vanbrabant AS, Boels LA, et al. (2003) Thinlayer liquid-based cervical cytology and PCR for detecting and typing human papillomavirus DNA in Flemish women. Br J Cancer 88: 560–566.
[23]
Grce M, Husnjak K, Magdic L, Ilijas M, Zlacki M, et al. (1997) Detection and typing of human papillomaviruses by polymerase chain reaction in cervical scrapes of Croatian women with abnormal cytology. Eur J Epidemiol 13: 645–651.
[24]
Huang LW, Chao SL, Chen PH, Chou HP (2004) Multiple HPV genotypes in cervical carcinomas: improved DNA detection and typing in archival tissues. J Clin Virol 29: 271–276.
[25]
Giraldo PC, Eleutério JRJ, Cavalcante DIM, Gon？alves AKS, Rom？o JAA, et al. (2008) The role of high-risk HPV-DNA testing in the male sexual partners of women with HPV-induced lesions. Eur J Obstet Gynecol Reprod Biol 137: 88–91.
[26]
Bleeker MCG, Hogewoning CJA, van Den Brule AJC, Voorhorst FJ, van Andel RE, et al. (2002) Penile lesions and human papillomavirus in male sexual partners of women with cervical intraepithelial neoplasia. J Am Acad Dermatol 47: 351–357.
[27]
Taner MZ, Taskiran C, Onan MA, Uluturky A, Himmetoglu O (2006) Genital human papillomavirus infection in the male sexual partners of women with isolated vulvar lesions. Int J Gynecol Cancer 16: 791–794.
[28]
Reiter PL, Pendergraft WF III, Brewer NT (2010) Meta-analysis of human papillomavirus infection concordance. Cancer Epidemiol Biomarkers Prev 11: 2916–2931.
[29]
Ramanakumar AV, Goncalves O, Richardson H, Tellier P, Ferenczy A, et al. (2010) Human papillomavirus (HPV) types 16, 18, 31, 45 DNA loads and HPV-16 integration in persistent and transient infections in young women. BMC Infectious Diseases 10: 326.
[30]
Giraldo PC, Gon？alves AK, Eleutério-Júnior J (2006) Secretory immunoglobulin A: A protective factor in the genital mucosa. Braz J Infect Dis 10: 232–234.
[31]
Ho GY, Bierman R, Beardsley L, Chang CJ, Burk RD (1998) Natural history of cervicovaginal papillomavirus infection young women. N Engl J Med 338: 423–428.
[32]
Parada R, Morales R, Giuliano AR, Cruz A, Castellsagué X, et al. (2011) Prevalence, concordance and determinants of human papillomavirus infection among heterosexual partners in a rural region in central Mexico. BMC Infect Dis 11: 25.
[33]
Widdice LE, Breland DJ, Jonte J, Farhat S, Ma Y, et al. (2010) Human papillomavirus concordance in heterosexual couples. J Adolesc Health 2: 151–159.
[34]
Giuliano AR, Palefsky JM, Goldstone S, Moreira Jr ED, Penny ME, et al. (2011) Efficacy of Quadrivalent HPV Vaccine against HPV Infection and Disease in Males. N Engl J Med 364: 401–411.
