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A Simple Method to Obtain Single Conidium Isolates Directly from Banana (Musa sp.) Leaves Infected with Mycosphaerella fijiensis Morelet

Keywords: black leaf streak, black sigatoka, conidia, asexual reproduction.

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Abstract:

a simple methodology to isolate conidia of mycosphaerella fijiensis from infected banana leaves was implemented. a method reported previously was laborious and required skilled trained personnel. here we describe a sampling method that consumes short periods of time and low costs and that can be carried out and reproduced by personnel with limited lab experience or expertise. it is practical and useful for extensive studies of foliar pathogen population and distribution.

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