Desarrollo y validación de una metodología para la determinación de residuos de orto-fenilfenol en aceite esencial de frutas cítricas en Tucumán, R. Argentina

the essential oils of citric fruits constitute complex mixtures of organic compounds which interfere with pesticide residues quantitation. as there exists scarce bibliography on ortho-phenylphenol (opp) determination in this commodity, the present work aims to adapt, develop and validate a simple and cheaper method for determining phenylphenol residues in citrus essential oil, while certifying it under iram 301 standard (iso/iec 17025:2005). citrus fruit analysis methods were used, taking advantage of opp acidity for its extraction from the commodity by simple acid-base extraction. later, gas chromatography with flame ionization detector (gc-fid) was used for detection and quantitation. average recoveries were the following: 89% to 104% within the range of 0.1 mg/kg to 100 mg/kg for lemon essential oil; 93% to 103% and 90% to 114%, for white and red grapefruit, respectively, within the range of 0.2 -10 mg/kg. detection limits were 0.05 mg/kg for lemon essential oil and 0.1 mg/kg for both white and red grapefruit essential oils. quantitation limits were 0.1 and 0.2 mg/kg for lemon and grapefruit essential oils, respectively. also, an acceptable linearity was observed in the evaluated ranges and relative uncertainties of 24%, 14% and 23% for lemon, white and red grapefruit essential oils, respectively, were obtained.