Perfil microbiológico de aislamientos en unidades neonatales en un hospital de tercer nivel de Bogotá, Colombia

objectives: investigating the prevalence and sensitivity of germs isolated from newborn in a referral hospital in bogotá. suggesting an empirical antibiotic treatment for neonatal infection. methods: cultures taken between february and december 2002 were analysed. blood cultures were processed using bact/alert (durham, nc), urine cultures by urocult (bio-bacter) and catheter tips in thioglycollate. bbl crystal identification system (bd, sparks, md) was used for identifying germs. antibiotic sensitivity was determined by disk diffusion. results: there were 1,097 positive aerobic and facultative aerobic germ cultures; 64,3% were gram-positive, 30,6% gram-negative and 4,9% were yeasts. gram-positive germs consisted of coagulase-negative staphylococci (64,2%), enterococcus (13,8%) and coagulase-positive staphylococci (13,3%). the most frequent gram-negatives were klebsiella (45,2%), eschericha coli (30,9%) and serratia (10,1%). staphylococcus epidermidis accounted for 64% of the coagulase-negative staphylococci. s. epidermidis susceptibility to vancomycin was 100%. coagulase-negative staphylococci susceptibility to rifampin and amikacin was 59% and 67,4% (respectively). coagulase-negative staphylococci resistance to beta-lactams was 86,4% (95% ci: 82,3-89,9). coagulase-positive staphylococci sensitivity to vancomycin was 100%. gram-negative susceptibility to imipenem was 98,1% (95% ci: 89,9-99,9), 78,1% to gentamicin (95% ci: 64,9-88,2) and 46,6% to amikacin (95% ci: 28,3-65,7). conclusions: there was high coagulase-negative staphylococci prevalence in neonatal infection (particularly s. epidermidis). all s. epidermidis and coagulase-positive staphylococci were sensitive to vancomycin. there was increasing coagulase-negative staphylococci and gram-negative resistance to oxacillin and amikacin, respectively.