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OALib Journal期刊
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Comparación entre Testsimplets? y Diff-Quik para la evaluación de la morfología espermática

Keywords: testsimplets?, diff-quik, sperm morphology, kruger strict criteria, peroxidase test, leukocytospermia.

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Abstract:

objective: to compare sperm morphology by testsimplets? and diff-quik staining. to compare the efficiency of staining cellular peroxidase and testsimplets? to assess leukocytes in semen. methods: we compared sperm morphology of 30 infertile patients evaluated by testsimplets? and diff-quik staining. additionally, in patients with more than 106 round cells per ml semen (n=7), we compared the assessment of leukocytes by staining cellular peroxidase and testsimplets?. setting: fertility clinic unifertes, in caracas, venezuela. results: there was no significant difference in the percentage of morphologically normal sperm, according to kruger?s strict criteria, between both stains. we found significant differences in the percentages of the following sperm morphological defects between staining: pyriform head, folded middle piece, irregular and presence of cytoplasmic droplets. there was no significant difference in the assessment of leukocytes in semen by staining cellular peroxidase and testsimplets?. conclusions: testsimplets? is practical for saving time and consequently, improving the efficiency in the delivery time of results. nonetheless, it is considerably more expensive than diff-quik.

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