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Ensayo inmunoenzimático en fase sólida para la detección de antígenos de Giardia lamblia

Keywords: giardia lamblia [immunology], antigens, protozoan, enzyme-linked immunosorbent assay [methods], faeces [parasitology].

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Abstract:

an elisa sandwich for the detection of giardia lamblia antigens in human faeces was standardized. 175 samples were studied: 77 positive, 61 negative to cysts and/or trophozoites by direct faeces test, and 19 positive to other parasite different from g. lamblia. the sensitivity of the technique was 94,8 % and the specificity 98,3 %. the method detects an antigen concentration of 31 ng. the procedure is simple, sensitive and specific so, it may be useful for diagnosis and in epidemiological studies.

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