Expresión en Escherichia coli del gen gspd l del sistema de secreción tipo II de Leptospira borgpetersenii serovariedad hardjo*

a fragment of 1.539 pb of the gene homologous to gspd of hardjobovis was clonated in the pet28a vector and it was transformed into e. coli c43 and rosetta strains. the sequence of gspdl showed 46 % of similitude with e. coli gspd secretin. the expression of recombinant gspdl was obtained in rosetta strain.