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Separación de fosfolípidos mediante la aplicación de fuerzas centrífugas

Keywords: centrifugation, phospholipids.

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Abstract:

introduction: centrifuge separation is very useful for obtaining pharmaceutical and biotechnological products. one of these applications is the separation and/or concentration of phospholipids. objective: to evaluate the use of different centrifuge separators, as well as the establishment of their operation conditions in order to obtain a typical phospholipidic mixture concentrate. methods: a physical characterization of the suspension to be treated was made for selecting the most adequate kind of centrifuge and its operational conditions were determined. the composition of the phospholipidic mixtures in every case was also evaluated results: both the disk and the tubular centrifuges turned to be fit for reaching the phospholipids concentration; hence the composition can be used for the formulation of liposomes, cosmetics and drugs. conclusions: given the adequacy of both centrifuges for reaching a specific phospholipidic mixture and their similar performances in the separation process, the best choice will be the vertical tubular centrifuge since it is less expensive and simpler in operation than the disk centrifuge.

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