Capacidad de las células Hep-2, Hep-2000？ Inmunofluorescencia y Hep-2000？ Colorzyme, en la determinación de ANAS y SSA/Ro en la evaluación inicial en pacientes con Enfermedad del Tejido Conectivo no Diferenciada

introduction: the presence of antinuclear antibodies (anas) has been traditionally associated to conective tissue diseases. and their determination is an important tool in the diagnose of these entities. in the last years the cells hep-2 has been used .in the inmunofluoresencia technique (ifi),. new cellular sustratos has been generated by genetic manipulation, denominated hep-2000？. these allow the identification of the antigen ro in the same procedure. the recent incorporation of enzymatic methods for the reading of the test, has generated a new technique called colorzyme, this allows the use of the microscope of light that become the procedure in an economical and functional alternative in comparison with the conventional ifi. objective: the present study pretend to establish the capacity of detection of anas in sustratos: hep-2 and hep-2000？ for ifi and colorzyme, in a group of patients with no differentiated conective tissue disease (ndctd). additionally compares the detection of the antigen ro in hep-2000？ cells and confront the results obtained by the enas-elisa technique . materials and methods: the presence of anas were analyzed in the serum of 26 patients with ndctd by the techniques: hep-2 ifi; hep-2000？ ifi; hep-2000 colorzyme？ and enas for elisa screening (with specificity for the auto antigens sm, rnp, ss-a/ro, ss-b/la, scl-70 and jo-1). results: hep-2000？: demonstrated a better capacity in order to found anas: 23 (88%) for ifi and 21 (81%) for colorzyme, compared with 20 (76%) of hep-2 ifi. all the patterns ？classic？ ifi was represented in the hep-2 cells ifi; hep-2000 ifi the homogeneous pattern was not observed. in colozyme tecnique the nucleolar and citoplasmic patron was not observed. in all the techniques the predominant form of presentation was the speckle-fine pattern: 50% colorzyme, 42.9% for hep-2000？ ifi and 34.6% for hep-2 ifi good correlations were obtained in the results among the technique hep-2000？ ifi and the colorzyme (r=0.74 p <0.000