Evaluación de la capacidad de desarrollo in vitro de ovocitos bovinos provenientes de vacas con predominancia fenotípica Bos taurus y Bos indicus

the aim of this study was to evaluate the in vitro development capacity of bovine oocytes from crossbred b. taurus and b. indicus cows. oocytes from bovine cows were collected from commercial slaughterhouse. the cumulus-oocyte complex (coc) ovaries were obtained by slicing technique, selecting those oocytes that had 2 to 3 layers of cumulus cells and homogeneous cytoplasm. after selection oocytes proceed with maturation (ivm) and fertilization in vitro (ivf). it used semen from a pure brahman bull (b. indicus). for the assessment of ivm as for ivf oocytes were fixed for about 24 hours at 4°c in methanol-acetic acid (3:1) solution and stained with 1.1% aceto-orcein. the maturation rate of oocytes from cows with b. indicus phenotypic predominance was 66.17%, whereas cows with b. taurus phenotypic predominance 50.94% (p>0.05). fertilization rate obtained in b. indicus phenotypic predominance group was 14.28% of oocytes normal penetrated and abnormal penetrated 35.72%, for cows with a phenotypic predominance b. taurus oocytes normal penetrated were 10.22% and 19.31% of abnormal oocytes penetrated. in conclusion, the present results indicate that oocytes from cows with phenotypic predominance b. indicus are more competent in the early stages of development in vitro than oocytes from cows with phenotypic predominance b. taurus.