Análisis morfométrico y ultraestructural del efecto del láser infrarrojo 10 j/cm2 sobre fibroblastos de conejo

rabbit temporo-mandibular joint sample tissue for control and asga laser exposition were obtained. the irradiation protocol consisted on doses of 10 j/cm2 emitted at 904 nm for periods of 5 minutes on daily basic through 15 consecutive days. later these samples were processed for trasmission electron microscopy (tem). micrographs from control and irradiated fibroblasts were obtained at 11,500 x magnification. these micrographs were submitted to morphometric measurements in order to investigate nuclear, cytoplasmic, rer and eu-heterochromatin fraction volume. their surface and nuclear-cytoplasmic ratio was also quantified. a significant difference was found between volumes of quantified cell components from normal and irradiated fibroblasts. on this basis it is possible to conclude that irradiated fibroblasts suffer a dose dependant drastic transformation on their ultrastructure, especially on their morphology and functionality which is related to the process of collagen synthesis and secretion.