Actividad antibacteriana de lactoferrina bovina y lactoferrina porcina sobre Escherichia Coli K88+

the antibacterial effect of bovine lactoferrin (lf) towards escherichia coli k88+ (e. coli k88+) was compared with that of porcine lf. e. coli k88+ is one of the main etiological agents of piglet diarrhea in the northern hemisphere. lactoferrins (lfs) were purified by ion exchange chromatography and further analyzed by electrophoresis using 8% sodium dodecyl sulfate polyacrylamide gel (sds-page) and immuno detection with anti-lactoferrin antibodies. bacteriostatic effect was assayed using 0.5 and 1.0 mg/ml of holo-lf (iron saturated) or apo-lf (iron free). in all test the holo-lfs showed negligible bacteriostatic activity while for apo-lfs the bovine protein had the highest activity. bactericide activity was assayed using bovine or porcine lf concentrations of 2.0, 4.0, 6.0 y 8.0 mg/ml. bovine lf had bactericide activity at 8.0 mg/ml while no growth inhibition was observed with porcine lf. these results suggest that bovine lf may serve in the prophylaxis of piglets？ diarrhea.