Desinfección y selección de inóculo in vitro de Abies religiosa

abies religiosa forests in ajusco (mexico) are in declination. it is required to restore populations using some survivor trees from affected slopes. the aims were to evaluate in vitro environmental for aseptic establishment of seeds and select the ad hoc explants for plantlet production in a. religiosa. disinfectants were tested (h2o2, c2h5oh, naocl) for in vitro germination. explants were evaluated (complete seed, complete or half -transversal cut- isolated embryo, and cotyledons and first leaves from in vitro germinated plantlets) for their in vitro establishment. the best treatment for a. religiosa seed disinfection is dips it in 3% v/v h2o2 and shakes 24 h. the best explants for in vitro propagation were complete seed and first leaves.