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Caracterización electroforética y cromatográfica del veneno del alacrán Rhopalurus junceus

Keywords: scorpion venoms, chromatography, electrophoresis.

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Abstract:

background: the blue scorpion venom, rhopalurus junceus is currently marketed with the name of escozul. this natural product is used in the treatment of different pathologies; however in the revised literature doesn't appear concerning information to the biochemical characterization of the extract of this cuban specimen. objective: to make a preliminary biochemical characterization on the crude venom of the cuban scorpion by means of the employment of two techniques dedicated to these purposes. method: the discontinuous electrophoresis of proteins was used in polyacrylamide to 15% and the chromatography of high pressure. results: a diffuse pattern corresponding to inferior size peptides to fourteen kda was identified, while for hplc thirteen peaks were determined in the crude venom of this species. conclusions: the blue scorpion venom presents a similar molecular composition to the one described for other species with an inferior molecular mixture to fourteen kda.

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