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Plant Methods

ISSN Print: 1746-4811

ISSN Online:

Website: http://www.plantmethods.com

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Synchronous high-resolution phenotyping of leaf and root growth in Nicotiana tabacum over 24-h periods with GROWMAP-plant

Ruts Tom,Matsubara Shizue,Walter Achim

Novel scanning procedure enabling the vectorization of entire rhizotron-grown root systems

Lobet Guillaume,Draye Xavier

Advances in plant gene-targeted and functional markers: a review

Poczai Péter,Varga Ildikó,Laos Maarja,Cseh András

Rhizo-lysimetry: facilities for the simultaneous study of root behaviour and resource use by agricultural crop and pasture systems

Eberbach Philip L,Hoffmann Jeffrey,Moroni Sergio J,Wade Leonard J

Striga parasitizes transgenic hairy roots of Zea mays and provides a tool for studying plant-plant interactions

Steven Runo, Sarah Macharia, Amos Alakonya, Jesse Machuka, Neelima Sinha, Julie Scholes

Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis

Zhong-Hua Chen, Cornelia Eisenach, Xin-Qin Xu, Adrian Hills, Michael R. Blatt

Evaluation of alternative RNA labeling protocols for transcript profiling with Arabidopsis AGRONOMICS1 tiling arrays

Marlen Müller, Andrea Patrignani, Hubert Rehrauer, Wilhelm Gruissem, Lars Hennig

Precursor uptake assays and metabolic analyses in isolated tomato fruit chromoplasts

Djédoux Angaman, Rocco Petrizzo, Francesc Hernández-Gras, Carmen Romero-Segura, Irene Pateraki, Montserrat Busquets, Albert Boronat

Xylem sap collection and extraction methodologies to determine in vivo concentrations of ABA and its bound forms by gas chromatography-mass spectrometry (GC-MS)

Andrew G Netting, Julian C Theobald, Ian C Dodd

In planta assays involving epigenetically silenced genes reveal inhibition of cytosine methylation by genistein

Sachiko Arase, Megumi Kasai, Akira Kanazawa

Identifying biological landmarks using a novel cell measuring image analysis tool: Cell-o-Tape

Andrew P French, Michael H Wilson, Kim Kenobi, Daniela Dietrich, Ute Vo？, Susana Ubeda-Tomás, Tony P Pridmore, Darren M Wells

Isolation of dimorphic chloroplasts from the single-cell C4 species Bienertia sinuspersici

Shiu-Cheung Lung, Makoto Yanagisawa, Simon DX Chuong

A novel procedure for absolute real-time quantification of gene expression patterns

Yingqing Lu, Lulu Xie, Jiani Chen

A simple and efficient method for the long-term preservation of plant cell suspension cultures

Anne-Marie Boisson, Elisabeth Gout, Richard Bligny, Corinne Rivasseau

PhosphoRice: a meta-predictor of rice-specific phosphorylation sites

Shufu Que, Kuan Li, Min Chen, Yongfei Wang, Qiaobin Yang, Wenfeng Zhang, Baoqian Zhang, Bangshu Xiong, Huaqin He

A rice transient assay system identifies a novel domain in NRR required for interaction with NH1/OsNPR1 and inhibition of NH1-mediated transcriptional activation

Mawsheng Chern, Wei Bai, Wing Hoi Sze-To, Patrick E Canlas, Laura E Bartley, Pamela C Ronald

A convenient method for simultaneous quantification of multiple phytohormones and metabolites: application in study of rice-bacterium interaction

Hongbo Liu, Xianghua Li, Jinghua Xiao, Shiping Wang

Hyperspectral imaging for small-scale analysis of symptoms caused by different sugar beet diseases

Anne-Katrin Mahlein, Ulrike Steiner, Christian Hillnhütter, Heinz-Wilhelm Dehne, Erich-Christian Oerke

Robust reconstitution of active cell-cycle control complexes from co-expressed proteins in bacteria

Hirofumi Harashima, Arp Schnittger

FSTVAL: a new web tool to validate bulk flanking sequence tags

Joung Sug Kim, Jiye Kim, Tae-Ho Lee, Kyong Mi Jun, Tea Hoon Kim, Yul-Ho Kim, Hyang-Mi Park, Jong-Seong Jeon, Gynheung An, Ung-Han Yoon, Baek Hie Nahm, Yeon-Ki Kim

A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

Jana Svacinova, Ondrej Novak, Lenka Plackova, Rene Lenobel, Josef Holik, Miroslav Strnad, Karel Dolezal

Poinsettia protoplasts - a simple, robust and efficient system for transient gene expression studies

Andrea Pitzschke, Helene Persak

Use of ex vitro composite plants to study the interaction of cowpea (Vigna unguiculata L.) with the root parasitic angiosperm Striga gesnerioides

Karolina E Mellor, Ava M Hoffman, Michael P Timko

A new method to identify flanking sequence tags in chlamydomonas using 3’-RACE

Laurence Meslet-Cladière, Olivier Vallon

The Arabidopsis thaliana-Alternaria brassicicola pathosystem: A model interaction for investigating seed transmission of necrotrophic fungi

Stephanie Pochon, Emmanuel Terrasson, Thomas Guillemette, Beatrice Iacomi-Vasilescu, Sonia Georgeault, Marjorie Juchaux, Romain Berruyer, Isabelle Debeaujon, Philippe Simoneau, Claire Campion

Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry

Kenneth W Berendzen, Maik B？hmer, Niklas Wallmeroth, Sébastien Peter, Marko Vesi？, Ying Zhou, Franziska KE Tiesler, Frank Schleifenbaum, Klaus Harter

Robust transformation procedure for the production of transgenic farmer-preferred cassava landraces

Ima Zainuddin, Kim Schlegel, Wilhelm Gruissem, Herve Vanderschuren

A novel fluorescent assay for sucrose transporters

Peter J Gora, Anke Reinders, John M Ward

Targeted parallel sequencing of large genetically-defined genomic regions for identifying mutations in Arabidopsis

Kun-hsiang Liu, Matthew McCormack, Jen Sheen

Plant lighting system with five wavelength-band light-emitting diodes providing photon flux density and mixing ratio control

Yano Akira,Fujiwara Kazuhiro

FACS-based purification of Arabidopsis microspores, sperm cells and vegetative nuclei

Borges Filipe,Gardner Rui,Lopes Telma,Calarco Joseph P

A high throughput DNA extraction method with high yield and quality

Xin Zhanguo,Chen Junping

A simple and efficient transient transformation for hybrid aspen (Populus tremula × P. tremuloides)

Takata Naoki,Eriksson Maria E

A novel xylogenic suspension culture model for exploring lignification in Phyllostachys bamboo

Ogita Shinjiro,Nomura Taiji,Kishimoto Takao,Kato Yasuo

CalloseMeasurer: a novel software solution to measure callose deposition and recognise spreading callose patterns

Zhou Ji,Spallek Thomas,Faulkner Christine,Robatzek Silke

Simple and efficient methods to generate split roots and grafted plants useful for long-distance signaling studies in Medicago truncatula and other small plants

Kassaw Tessema K,Frugoli Julia A

Development of a rapid and inexpensive method to reveal natural antisense transcripts

Collani Silvio,Barcaccia Gianni

Rapid quantification of plant-powdery mildew interactions by qPCR and conidiospore counts

We？ling Ralf,Panstruga Ralph

PhenoPhyte: a flexible affordable method to quantify 2D phenotypes from imagery

Green Jason M,Appel Heidi,Rehrig Erin MacNeal,Harnsomburana Jaturon

Barley callus: a model system for bioengineering of starch in cereals

Carciofi Massimiliano,Blennow Andreas,Nielsen Morten M,Holm Preben B

OSCILLATOR: A system for analysis of diurnal leaf growth using infrared photography combined with wavelet transformation

Bours Ralph,Muthuraman Manickam,Bouwmeester Harro,van der Krol Alexander

Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system

Tuttle John,Haigler Candace H,Robertson Dominique

Protocol: Chromatin immunoprecipitation (ChIP) methodology to investigate histone modifications in two model diatom species

Lin Xin,Tirichine Le？la,Bowler Chris

A cost-effective method for Illumina small RNA-Seq library preparation using T4 RNA ligase 1 adenylated adapters

Chen Yun-Ru,Zheng Yi,Liu Bao,Zhong Silin

Methods of staining and visualization of sphingolipid enriched and non-enriched plasma membrane regions of Arabidopsis thaliana with fluorescent dyes and lipid analogues

Blachutzik J？rg O,Demir Fatih,Kreuzer Ines,Hedrich Rainer

Protocol: High-throughput and quantitative assays of auxin and auxin precursors from minute tissue samples

Liu Xing,Hegeman Adrian D,Gardner Gary,Cohen Jerry D

Inflorescence stem grafting made easy in Arabidopsis

Nisar Nazia,Verma Shelley,Pogson Barry J,Cazzonelli Christopher I

Universal Multiplex PCR: a novel method of simultaneous amplification of multiple DNA fragments

Wen Daxing,Zhang Chunqing

Phytotracker, an information management system for easy recording and tracking of plants, seeds and plasmids

Nieuwland Jeroen,Sornay Emily,Marchbank Angela,de Graaf Barend HJ

Concurrent profiling of indole-3-acetic acid, abscisic acid, and cytokinins and structurally related purines by high-performance-liquid-chromatography tandem electrospray mass spectrometry

Farrow Scott C,Emery RJ Neil

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