Microbial Cell Factories - oalib

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Microbial Cell Factories

ISSN Print: 1475-2859

ISSN Online:

主页: http://www.microbialcellfactories.com

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Bacterial diversity and reductive dehalogenase redundancy in a 1,2-dichloroethane-degrading bacterial consortium enriched from a contaminated aquifer

Massimo Marzorati, Annalisa Balloi, Francesca de Ferra, Lorenzo Corallo, Giovanna Carpani, Lieven Wittebolle, Willy Verstraete, Daniele Daffonchio

Hyaluronic acid production by Streptococcus zooepidemicus in marine by-products media from mussel processing wastewaters and tuna peptone viscera

José A Vázquez, María I Montemayor, Javier Fraguas, Miguel A Murado

Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory

Cecilia Ferndahl, Nicklas Bonander, Christel Logez, Renaud Wagner, Lena Gustafsson, Christer Larsson, Kristina Hedfalk, Richard AJ Darby, Roslyn M Bill

Metabolic regulation of Escherichia coli and its gdhA, glnL, gltB, D mutants under different carbon and nitrogen limitations in the continuous culture

Rahul Kumar, Kazuyuki Shimizu

Reduction of oxidative cellular damage by overexpression of the thioredoxin TRX2 gene improves yield and quality of wine yeast dry active biomass

Rocío Gómez-Pastor, Roberto Pérez-Torrado, Elisa Cabiscol, Joaquim Ros, Emilia Matallana

A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

Mirja Krause, Kaisa Ukkonen, Tatu Haataja, Maria Ruottinen, Tuomo Glumoff, Antje Neubauer, Peter Neubauer, Antti Vasala

Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus

Saul N Rocha, José Abrah？o-Neto, María E Cerdán, María I González-Siso, Andreas K Gombert

Isolation, characterization and heterologous expression of a novel chitosanase from Janthinobacterium sp. strain 4239

Mads G Johnsen, Ole C Hansen, Peter Stougaard

Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into Streptomyces ansochromogenes lead to an improved nikkomycin production

Guojian Liao, Jine Li, Lei Li, Haihua Yang, Yuqing Tian, Huarong Tan

Cloning of the Zygosaccharomyces bailii GAS1 homologue and effect of cell wall engineering on protein secretory phenotype

Simone Passolunghi, Luca Riboldi, Laura Dato, Danilo Porro, Paola Branduardi

Differential proteomic analysis highlights metabolic strategies associated with balhimycin production in Amycolatopsis balhimycina chemostat cultivations

Giuseppe Gallo, Rosa Alduina, Giovanni Renzone, Jette Thykaer, Linda Bianco, Anna Eliasson-Lantz, Andrea Scaloni, Anna Puglia

PGM2 overexpression improves anaerobic galactose fermentation in Saccharomyces cerevisiae

Rosa Garcia Sanchez, B？rbel Hahn-H？gerdal, Marie F Gorwa-Grauslund

High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture

Ruidong Chen, Zhigang Zhou, Yanan Cao, Yingguo Bai, Bin Yao

Reduced evolvability of Escherichia coli MDS42, an IS-less cellular chassis for molecular and synthetic biology applications

Kinga Umenhoffer, Tamás Fehér, Gabriella Balikó, Ferhan Ayaydin, János Pósfai, Frederick R Blattner, Gy？rgy Pósfai

Production and purification of staphylococcal nuclease in Lactococcus lactis using a new expression-secretion system and a pH-regulated mini-reactor

Nicolas Trémillon, Nicolas Issaly, Julien Mozo, Thomas Duvignau, Hervé Ginisty, Eric Devic, Isabelle Poquet

Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures

Juozas ？iurkus, Johanna Panula-Per？l？, Uwe Horn, Mario Kraft, Renata Rim？eliene, Peter Neubauer

Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin

Anna Zanfardino, Odile F Restaino, Eugenio Notomista, Donatella Cimini, Chiara Schiraldi, Mario De Rosa, Maurilio De Felice, Mario Varcamonti

A comparative summary of expression systems for the recombinant production of galactose oxidase

Oliver Spadiut, Lisbeth Olsson, Harry Brumer

Expression of an extremely acidic β-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence

Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo, Bin Yao

A novel prokaryotic vector for identification and selection of recombinants: Direct use of the vector for expression studies in E. coli

Sampali Banerjee, Jitendra Kumar, Anjali Apte-Deshpande, Sriram Padmanabhan

Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification

Shuang Zhang, Zhi Wang, Guo Chen

Towards universal systems for recombinant gene expression

Hans S？rensen

Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B1

Doris Hartinger, Stefan Heinl, Heidi Schwartz, Reingard Grabherr, Gerd Schatzmayr, Dietmar Haltrich, Wulf-Dieter Moll

Production of glycoprotein vaccines in Escherichia coli

Julian Ihssen, Michael Kowarik, Sandro Dilettoso, Cyril Tanner, Michael Wacker, Linda Th？ny-Meyer

Glucose uptake regulation in E. coli by the small RNA SgrS: comparative analysis of E. coli K-12 (JM109 and MG1655) and E. coli B (BL21)

Alejandro Negrete, Weng-Ian Ng, Joseph Shiloach

Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae

Irina Borodina, Bettina M Jensen, Ib S？ndergaard, Lars K Poulsen

Isolation of cell-free bacterial inclusion bodies

Escarlata Rodríguez-Carmona, Olivia Cano-Garrido, Joaquin Seras-Franzoso, Antonio Villaverde, Elena García-Fruitós

Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains

Philipp H？fer, Young J Choi, Michael J Osborne, Carlos B Miguez, Patrick Vermette, Denis Groleau

Single cell oils of the cold-adapted oleaginous yeast Rhodotorula glacialis DBVPG 4785

Alberto Amaretti, Stefano Raimondi, Maurizio Sala, Lucia Roncaglia, Marzia De Lucia, Alan Leonardi, Maddalena Rossi

Fed-batch process for the psychrotolerant marine bacterium Pseudoalteromonas haloplanktis

Boris Wilmes, Angelika Hartung, Michael Lalk, Manuel Liebeke, Thomas Schweder, Peter Neubauer

Isolation of biologically active nanomaterial (inclusion bodies) from bacterial cells

？pela Peternel, Radovan Komel

Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin

Chandrasekhar Gurramkonda, Sulena Polez, Natasa Skoko, Ahmad Adnan, Thomas G？bel, Dipti Chugh, Sathyamangalam Swaminathan, Navin Khanna, Sergio Tisminetzky, Ursula Rinas

Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis

Andrew S Wieczorek, Vincent JJ Martin

Two distinct regions in the model protein Peb1 are critical for its heterologous transport out of Escherichia coli

Lena Anton, Katariina Majander, Harri Savilahti, Liisa Laakkonen, Benita Westerlund-Wikstr？m

Biosynthesis of chiral 3-hydroxyvalerate from single propionate-unrelated carbon sources in metabolically engineered E. coli

Hsien-Chung Tseng, Catey L Harwell, Collin H Martin, Kristala LJ Prather

Antibiotic-free selection in E. coli: new considerations for optimal design and improved production

Isabelle Peubez, Nicolas Chaudet, Charlotte Mignon, Géraldine Hild, Stéphanie Husson, Virginie Courtois, Karelle De Luca, Denis Speck, Régis Sodoyer

Protein solubility and differential proteomic profiling of recombinant Escherichia coli overexpressing double-tagged fusion proteins

Chung-Hsien Cheng, Wen-Chien Lee

Physiological characteristics of the extreme thermophile Caldicellulosiruptor saccharolyticus: an efficient hydrogen cell factory

Karin Willquist, Ahmad A Zeidan, Ed WJ van Niel

Metabolic response of Geobacter sulfurreducens towards electron donor/acceptor variation

Tae Hoon Yang, Maddalena V Coppi, Derek R Lovley, Jun Sun

The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate

Xin Lu, Jibin Sun, Manfred Nimtz, Josef Wissing, An-Ping Zeng, Ursula Rinas

An antibiotic, heavy metal resistant and halotolerant Bacillus cereus SIU1 and its thermoalkaline protease

Sanjay K Singh, Vinayak R Tripathi, Rakesh K Jain, Surendra Vikram, Satyendra K Garg

Global expression studies in baker's yeast reveal target genes for the improvement of industrially-relevant traits: the cases of CAF16 and ORC2

Roberto Pérez-Torrado, Joaquín Panadero, María Hernández-López, José Prieto, Francisca Randez-Gil

Automated assay for screening the enzymatic release of reducing sugars from micronized biomass

David Navarro, Marie Couturier, Gabriela da Silva, Jean-Guy Berrin, Xavier Rouau, Marcel Asther, Christophe Bignon

Identification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms

Chengjian Jiang, Lan-Lan Wu, Gao-Chao Zhao, Pei-Hong Shen, Ke Jin, Zhen-Yu Hao, Shuang-Xi Li, Ge-Fei Ma, Feng-Feng Luo, Guo-Qing Hu, Wen-Long Kang, Xing-Mei Qin, You-Li Bi, Xian-Lai Tang, Bo Wu

Dissection of an old protein reveals a novel application: domain D of Staphylococcus aureus Protein A (sSpAD) as a secretion - tag

Thomas Heel, Michael Paal, Rainer Schneider, Bernhard Auer

New method for selection of hydrogen peroxide adapted bifidobacteria cells using continuous culture and immobilized cell technology

Valeria Mozzetti, Franck Grattepanche, Déborah Moine, Bernard Berger, Enea Rezzonico, Leo Meile, Fabrizio Arigoni, Christophe Lacroix

The genome-scale metabolic network analysis of Zymomonas mobilis ZM4 explains physiological features and suggests ethanol and succinic acid production strategies

Kyung Lee, Jong Park, Tae Kim, Hongseok Yun, Sang Lee

Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein

Shardul S Salunkhe, Veena A Raiker, Sachin Rewanwar, Prakash Kotwal, Avijeet Kumar, Sriram Padmanabhan

Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst

Sandra Abad, Jozef Nahalka, Gabriele Bergler, S Alison Arnold, Robert Speight, Ian Fotheringham, Bernd Nidetzky, Anton Glieder

High yield expression of leptospirosis vaccine candidates LigA and LipL32 in the methylotrophic yeast Pichia pastoris

Daiane D Hartwig, Thaís L Oliveira, Fabiana K Seixas, Karine M Forster, Caroline Rizzi, Cláudia P Hartleben, Alan JA McBride, Odir A Dellagostin

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