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ISSN Print: 1750-2799

ISSN Online:

Website: https://www.nature.com/nprot

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A practical guide to optimization in X10 expansion microscopy

Using CAPTURE to detect spacer acquisition in native CRISPR arrays

Nongenetic optical neuromodulation with silicon-based materials

Guidelines for using sigQC for systematic evaluation of gene signatures

Whole-brain block-face serial microscopy tomography at subcellular resolution using FAST

Facile 18F labeling of non-activated arenes via a spirocyclic iodonium(III) ylide method and its application in the synthesis of the mGluR5 PET radiopharmaceutical [18F]FPEB

Regeneration of a bioengineered 3D integumentary organ system from iPS cells

RNase A treatment and reconstitution with DNA damage response RNA in living cells as a tool to study the role of non-coding RNA in the formation of DNA damage response foci

Nanoscale chemical imaging using tip-enhanced Raman spectroscopy

Detection and quantification of RNA decay intermediates using XRN1-resistant reporter transcripts

High-resolution, strand-specific R-loop mapping via S9.6-based DNA–RNA immunoprecipitation and high-throughput sequencing

Induction of neoantigen-reactive T cells from healthy donors

Engineering proteins for allosteric control by light or ligands

Using Cre-recombinase-driven Polylox barcoding for in vivo fate mapping in mice

Purification of tubulin with controlled post-translational modifications by polymerization–depolymerization cycles

Standardization of complex biologically derived spectrochemical datasets

Synthetic chemistry in water: applications to peptide synthesis and nitro-group reductions

Derivation of enteric neuron lineages from human pluripotent stem cells

Fabrication and practical applications of molybdenum disulfide nanopores

Asymmetric-flow field-flow fractionation technology for exomere and small extracellular vesicle separation and characterization

INFOGEST static in vitro simulation of gastrointestinal food digestion

Holographic two-photon activation for synthetic optogenetics

Protocol Update for large-scale genome and gene function analysis with the PANTHER classification system (v.14.0)

Fractionation of lignocellulosic biomass to produce uncondensed aldehyde-stabilized lignin

Creation and analysis of biochemical constraint-based models using the COBRA Toolbox v.3.0

RNA sequencing and swarm intelligence–enhanced classification algorithm development for blood-based disease diagnostics using spliced blood platelet RNA

Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED–FCS

Using the tube test to measure social hierarchy in mice

Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue

High-speed imaging of glutamate release with genetically encoded sensors

R-ChIP for genome-wide mapping of R-loops by using catalytically inactive RNASEH1

A nanostructure platform for live-cell manipulation of membrane curvature

MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing

A non-enzymatic method for dissection of mouse bladder urothelial tissue

Temperature imaging using a cationic linear fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy

Quantitative imaging of sleep behavior in Caenorhabditis elegans and larval Drosophila melanogaster

Capturing 5？ and 3？ native ends of mRNAs concurrently with Akron sequencing

High-resolution 3D imaging of fixed and cleared organoids

Mass spectrometry–based identification of MHC-bound peptides for immunopeptidomics

High-dimensional cell-level analysis of tissues with Ce3D multiplex volume imaging

Real-time microscopy and physical perturbation of bacterial pili using maleimide-conjugated molecules

Bioluminescence resonance energy transfer–based imaging of protein–protein interactions in living cells

Microfabricated blood vessels for modeling the vascular transport barrier

Isolating the human cochlea to generate bone powder for ancient DNA analysis

High-resolution 3D imaging and analysis of axon regeneration in unsectioned spinal cord with or without tissue clearing

Nonviral ultrasound-mediated gene delivery in small and large animal models

Microfluidic reprogramming to pluripotency of human somatic cells

A comprehensive pipeline for translational top-down proteomics from a single blood draw

Application of anisotropic NMR parameters to the confirmation of molecular structure

Differential viral accessibility (DIVA) identifies alterations in chromatin architecture through large-scale mapping of lentiviral integration sites

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