Added Value of 50-Gene Panel Sequencing to Distinguish Multiple Primary Lung Cancers from Pulmonary Metastases: A Systematic Investigation

Alexandra ten Heuvel,Danielle A.M. Heideman,Gerarda J.M. Herder,J. Alain Kummer,Karen C. Scheidel,Kees A. Seldenrijk,Paul Roepman,Tobias Sprong

Droplet Digital PCR Is a Robust Tool for Monitoring Minimal Residual Disease in Adult Philadelphia-Positive Acute Lymphoblastic Leukemia

Angela Minervini,Antonella Zagaria,Claudia Brunetti,Cosimo Cumbo,Crescenzio F. Minervini,Elisa Parciante,Francesco Albano,Giorgina Specchia,Giuseppina Tota,Luciana Impera,Luisa Anelli,Nicoletta Coccaro,Paola Carluccio,Paola Casieri,Paola Orsini

Suppression of Wild-Type Amplification by Selectivity Enhancing Agents in PCR Assays that Utilize SuperSelective Primers for the Detection of Rare Somatic Mutations

Diana Y. Vargas,Fred R. Kramer,Salvatore A.E. Marras,Sanjay Tyagi

External Quality Assessment Identifies Training Needs to Determine the Neoplastic Cell Content for Biomarker Testing

Cleo Keppens,Elisabeth M.C. Dequeker,Gert De Hertogh,J. Han van Krieken,Kelly Dufraing,Véronique Tack

Validation and Implementation of BRCA1/2 Variant Screening in Ovarian Tumor Tissue

Astrid Baalbergen,Christi J. van Asperen,Dina Ruano,Judith R. Kroep,Juul T. Wijnen,Katja N. Gaarenstroom,Maaike P.G. Vreeswijk,Maartje Nielsen,Marjolein J. Kagie,Marthe M. de Jonge,Monique E.M.M. Bos,Natalja T. ter Haar,Nienke van der Stoep,Ronald van Eijk,Tjalling Bosse,Tom van Wezel,Vincent T.H.B.M. Smit

Development and Evaluation of a Pan-Sarcoma Fusion Gene Detection Assay Using the NanoString nCounter Platform

Aly Karsan,Angela Goytain,Cheng-Han Lee,Kenneth T.E. Chang,Tony L. Ng,Torsten O. Nielsen,Tracy Tucker

Table of Contents

Multicenter Evaluation of the Idylla NRAS-BRAF Mutation Test in Metastatic Colorectal Cancer

Barbara Dockhorn-Dworniczak,Beatriz Bellosillo,Christiana Rossenbach,Clara Montagut,Claudia M. Luna-Aguirre,Federico Rojo,Francesca Castiglione,Hendrikus J. Dubbink,Hilla Tabibian-Keissar,Hugo A. Barrera-Salda？a,Iván Prieto-Potin,Jean-Christophe Sabourin,José C. Machado,José L. Costa,Karl-Friedrich Bürrig,Linea Melchior,Livia Ronchetti,Lotte Douglas-Berger,Matthew Evans,Matthew Smith,Michael Bennett,Mohammed Alorini,Rinat Yacobi,Simonetta Buglioni,Ulrike Cooper,Veronica Pennati,Werner Reiltin

A Highly Sensitive and Robust Method for Hepatitis B Virus Covalently Closed Circular DNA Detection in Single Cells and Serum

Er-Feng Yuan,Jing-Tao Huang,Mei-Yan Liao,Roy Morgan,Song-Mei Liu,Xia Li,Xing-Hui Liu,Yi-Min Hu,Ying Yang

Validation of a Customized Bioinformatics Pipeline for a Clinical Next-Generation Sequencing Test Targeting Solid Tumor–Associated Variants

Charles E. Hill,Geoffrey H. Smith,Linsheng Zhang,Michael R. Rossi,Thomas Schneider

Describing the Reportable Range Is Important for Reliable Treatment Decisions: A Multiple Laboratory Study for Molecular Tumor Profiling Using Next-Generation Sequencing

Cleo Keppens,Ed Schuuring,Elisabeth M.C. Dequeker,Jeroen Van Houdt,Karen Zwaenepoel,Lien Spans,Patrick Pauwels,Véronique Tack

Comprehensive Validation of Cytology Specimens for Next-Generation Sequencing and Clinical Practice Experience

Agnes Balla,Catherine E. Cottrell,Ken J. Hampel,Mukesh K. Sharma,Nikoletta Sidiropoulos

Curating Clinically Relevant Transcripts for the Interpretation of Sequence Variants

Ahmad N. Abou Tayoun,Andrea M. Oza,Andrew R. Grant,Brandon J. Cushman,Elizabeth Hynes,Heidi L. Rehm,Marina T. DiStefano,Mark J. Bowser,Michael A. Gonzalez,Rebecca K. Siegert,Sami S. Amr,Sarah E. Hemphill

Scientific Integrity Policy

Next-Generation Sequencing for Lymphomas: Perfecting a Pipeline for Personalized Pathobiologic and Prognostic Predictions

Adam Bagg,Andreas Rosenwald,Robert S. Ohgami

Abstracts of the 1st AMP Europe Congress

MYD88 Mutations and Sensitivity to Ibrutinib Therapy

Y. Lynn Wang

A Novel and Reliable Method to Detect Microsatellite Instability in Colorectal Cancer by Next-Generation Sequencing

Chenglin Liu,Chenhan Zhong,Dong Xu,Jinghong Xu,Lizhen Zhu,Wanglong Deng,Xuefeng Fang,Yanqin Huang,Ying Yuan

Evaluation of a Pan-Leishmania Spliced-Leader RNA Detection Method in Human Blood and Experimentally Infected Syrian Golden Hamsters

Dimitri Bulté,Dorien Mabille,Eline Eberhardt,Fabiana Alves,Guy Caljon,Jane Mbui,Lieselotte Van Bockstal,Louis Maes,Magali Van den Kerkhof,Paul Cos,Peter Delputte,Sarah Hendrickx,Séverine Monnerat

Efficient Detection of Copy Number Mutations in PMS2 Exons with a Close Homolog

Brian H. Shirts,Cecily P. Vaughn,Chang Liu,Christina Smith,Colin C. Pritchard,Daniel S. Herman,Selvi Palaniappan

A Reliable Targeted Next-Generation Sequencing Strategy for Diagnosis of Myopathies and Muscular Dystrophies, Especially for the Giant Titin and Nebulin Genes

Caroline Espil,Claude Cances,Corine Theze,Cyril Goizet,Delphine Lacourt,Dimitri Renard,Emmanuelle Uro-Coste,Eric Bieth,Fran？ois Rivier,Guilhem Sole,Henri Pegeot,Kevin Yauy,Marie-Christine Arné-Bes,Marie-Laure Martin Negrier,Michel Koenig,Mireille Claustres,Mireille Cossée,Pascal Cintas,Raul Juntas Morales,Reda Zenagui,Ulrike Walther-Louvier,Valérie Rigau,Xavier Ferrer-Monasterio

Molecular Minimal Residual Disease Monitoring in Acute Myeloid Leukemia: Challenges and Future Directions

Adrian G. Selim,Andrew S. Moore

Development of a Fluorescence in Situ Hybridization Probe for Detecting IKZF1 Deletion Mutations in Patients with Acute Lymphoblastic Leukemia

Chikara Shimizu,Daigo Hashimoto,Junichi Hashiguchi,Kaoru Kahata,Kohei Okada,Masahiro Onozawa,Masahisa Tsuji,Masao Nakagawa,Satoshi Oguri,Shinichi Fujisawa,Takanori Teshima,Takeshi Kondo

Development and Verification of an RNA Sequencing (RNA-Seq) Assay for the Detection of Gene Fusions in Tumors

Amber M. McDonald,Asha A. Nair,Benjamin R. Kipp,Brittany C. Thomas,Eric W. Klee,Gavin R. Oliver,Jadee L. Neff,Jaime I. Davila,Jennifer L. Winters,Jesse S. Voss,Jessica R. Balcom,Jin Jen,Kandelaria M. Rumilla,Kevin C. Halling,Long Jin,Numrah Fadra,Rebecca N. Wehrs,Robert B. Jenkins,Rondell P. Graham,Umut Aypar,Xianglin Wu

A Head-to-Head Analytical Comparison of Cobas 4800 HPV, PapilloCheck HPV Screening, and LMNX Genotyping Kit HPV GP for Detection of Human Papillomavirus DNA in Cervical and Cervicovaginal Swabs

Blazena Zborilova,Hana Jaworek,Ivana Oborna,Jana Brezinova,Jana Vrbkova,Jiri Drabek,Karel Huml,Marian Hajduch,Peter Vanek,Radim Marek,Vladimira Koudelakova

Optimal Reference Gene Selection for Expression Studies in Human Reticulocytes

Anu Aggarwal,Deepak Bansal,Ganesh K. Viswanathan,ManUpdesh S. Sachdeva,Manu Jamwal,Pankaj Malhotra,Prashant Sharma,Reena Das

Diagnostic Targeted Sequencing Panel for Hepatocellular Carcinoma Genomic Screening

Andrea Garofoli,Charlotte K.Y. Ng,Luca Quagliata,Luigi M. Terracciano,Manuela Lanzafame,Markus H. Heim,Matthias S. Matter,Nadia Tosti,Salvatore Piscuoglio,Stefan Wieland,Valeria Perrina,Viola Paradiso

Recommendations for Clinical CYP2C19 Genotyping Allele Selection: A Report of the Association for Molecular Pathology

Andria L. Del Tredici,Houda Hachad,Karen E. Weck,Lisa V. Kalman,Stuart A. Scott,Victoria M. Pratt,Yuan Ji

Reference Size Matching, Whole-Genome Amplification, and Fluorescent Labeling as a Method for Chromosomal Microarray Analysis of Clinically Actionable Copy Number Alterations in Formalin-Fixed, Paraffin-Embedded Tumor Tissue

Aditi Khurana,Cynthe L. Sims,Jayne Scoggin,Mathew W. Moore,Philip D. Cotter,Samuel Koo,Shailin Govender,Shelly R. Gunn

OncoKids: A Comprehensive Next-Generation Sequencing Panel for Pediatric Malignancies

Alex Ryutov,Alexander R. Judkins,Amanda L. Treece,Beverly B. Rogers,Chao Jie Zhen,David M. Parham,Dejerianne G. Ostrow,Dennis T. Maglinte,Florette K. Hazard,Gordana Raca,Jaclyn A. Biegel,James Done,Jeffrey M. Conroy,Jianling Ji,Jonathan Buckley,Joshua L. Deignan,Matthew C. Hiemenz,Moiz Bootwalla,Timothy J. Triche,Tracy M. Busse,Xiaowu Gai,Xinjie Xu

Editorial Board

Analytical Validation of Clinical Whole-Genome and Transcriptome Sequencing of Patient-Derived Tumors for Reporting Targetable Variants in Cancer

Alex Kentsis,Anne-Katrin Emde,Avinash Abhyankar,David Lin,Depinder Khaira,Dina Manaa,Dino Robinson,Esra Dikoglu,Ferrah London,Govind Bhagat,Heather Geiger,Kanika Arora,Kazimierz O. Wrzeszczynski,Lukasz Kozon,Mahesh M. Mansukhani,Michelle F. Lamendola-Essel,Minita Shah,Nicolas Robine,Olca Basturk,Umesh Bhanot,Vaidehi Jobanputra,Vanessa Felice,Xiaolan Fang

Perspectives on the Affordability of Precision Medicine

Daniel H. Farkas

Evaluation of Diagnostic Utility of a High-Risk Human Papillomavirus PCR Test on Formalin-Fixed, Paraffin-Embedded Head and Neck Tumor Tissues

Albert N. Huho,Nour Yadak,Shangxin Yang,Thèrése J. Bocklage

Evaluation of a Urine-Based Rapid Molecular Diagnostic Test with Potential to Be Used at Point-of-Care for Pulmonary Tuberculosis: Cape Town Cohort

Christof Geldmacher,Eric Rivera-Milla,Ines Labugger,Keertan Dheda,Krutarth Patel,Maria Wesolowski,Matilde Nagel,Michael Hoelscher,Stefan Dees

Editorial Board

Comprehensive Genomic Profiling of Malignant Effusions in Patients with Metastatic Lung Adenocarcinoma

Chieh-Yu Lin,Christian A. Kunder,Christina S. Kong,Gerald J. Berry,Henning Stehr,James L. Zehnder,Soo-Ryum Yang,Steven R. Long

Updated Molecular Testing Guideline for the Selection of Lung Cancer Patients for Treatment With Targeted Tyrosine Kinase Inhibitors: Guideline From the College of American Pathologists, the International Association for the Study of Lung Cancer, and the Association for Molecular Pathology

Benjamin Solomon,Carol Colasacco,Christina B. Ventura,Dara L. Aisner,David J. Kwiatkowski,Eric H. Bernicker,Erik Thunnissen,Fred R. Hirsch,Jan A. Nowak,Keith Kerr,Lesley H. Souter,Lynette Sholl,Marc Ladanyi,Maria E. Arcila,Mary Beth Beasley,Ming S. Tsao,Murry W. Wynes,Neal I. Lindeman,Philip T. Cagle,Robyn Temple-Smolkin,Sanja Dacic,Yasushi Yatabe

Editorial Board

Noncoding RNA Expression and Targeted Next-Generation Sequencing Distinguish Tubulocystic Renal Cell Carcinoma (TC-RCC) from Other Renal Neoplasms

Angela M. Araujo,Carla Sole,Charles H. Lawrie,Erika Larrea,Ibai Goicoechea,Isabel Alvarado-Cabrero,José I. López,Lorea Manterola,Maris Sperga,Marta Fernandez-Mercado,María Arestín,María Armesto,María M. Caffarel,Michal Michal,Ondrej Hes

Is Tubulocystic Renal Cell Carcinoma Real?: Genomic Analysis Confirms the World Health Organization Classification

Hiroshi Miyamoto,Paul G. Rothberg,Yi Ding

Navigating the Peer Review Process

Barbara Zehnbauer,Chhavi Chauhan,Emily H. Essex

Heterogeneity of BRAF, NRAS, and TERT Promoter Mutational Status in Multiple Melanomas and Association with MC1R Genotype: Findings from Molecular and Immunohistochemical Analysis

Ambra Antonini,Claudia Martorelli,Cristina Pellegrini,Gianluca Cipolloni,Ketty Peris,Laura Del Regno,Lucia Di Nardo,Maria Concetta Fargnoli,Maria Giovanna Maturo,Marina De Padova,Pietro Leocata,Sara Strafella,Tamara Micantonio

A Monochrome Multiplex Real-Time Quantitative PCR Assay for the Measurement of Mitochondrial DNA Content

Anthony Y.Y. Hsieh,David Deng,Hélène C.F. C？té,Izabella Gadawska,Matthew Budd

Evaluation of a Hepatitis C Virus Core Antigen Assay in Plasma and Dried Blood Spot Samples

Angelica Soker,Behzad Hajarizadeh,Beth Catlett,Camelia Quek,Danica Martinez,Fran？ois M.J. Lamoury,Gavin Cloherty,Gregory J. Dore,Janaki Amin,Jason Grebely,Philip Cunningham,Philippa Marks,Tanya L. Applegate

Molecular Analysis of Gene Fusions in Bone and Soft Tissue Tumors by Anchored Multiplex PCR–Based Targeted Next-Generation Sequencing

Anne-Marie Cleton-Jansen,Arjen H.G. Cleven,Dina Ruano,Judith V.M.G. Bovée,Karoly Szuhai,Suk Wai Lam,Tom van Wezel

Pre-Analytical Handling Conditions and Small RNA Recovery from Urine for miRNA Profiling

Alix Ashare,Bruce A. Stanton,Carol S. Ringelberg,David A. Armstrong,Haley F. Hazlett,John A. Dessaint,Louisa Howard,Mark A. Cervinski,Moemen A.K. Abdalla,Roxanna L. Barnaby

Analytical Validation of a Hybrid Capture–Based Next-Generation Sequencing Clinical Assay for Genomic Profiling of Cell-Free Circulating Tumor DNA

Allison Welsh,Alyssa Tsiros,Amy Donahue,Bernard Fendler,Daniel S. Lieber,Dean Pavlick,Doron Lipson,Eric Peters,Erica Schleifman,Garrett M. Frampton,Geneva Young,Geoff A. Otto,Jason D. Hughes,Jeffrey P. Gregg,Jeffrey S. Ross,Jie He,Jill M. Spoerke,Jon H. Chung,Lauren Young,Mandy Zhao,Mark Bailey,Mark Kennedy,Mark Lackner,Michael Coyne,Niru Chennagiri,Philip J. Stephens,Shan Zhong,Siraj M. Ali,Steven Gendreau,Steven Roels,Tariq Mughal,Timothy Brennan,Travis A. Clark,Vincent A. Miller,Virginia Breese

Multiple Ways to Detect IDH2 Mutations in Angioimmunoblastic T-Cell Lymphoma from Immunohistochemistry to Next-Generation Sequencing

Alexandra Traverse-Glehen,Ana？s Pujals,Anissa Moktefi,Aurélie Dupuy,Christiane Copie-Bergman,Corinne Haioun,Cyrielle Robe,Elsa Poullot,Fran？ois Lemonnier,Karima Mokhtari,Laurence de Leval,Marie C. Rousselet,Marie H. Delfau-Larue,Nadine Martin-Garcia,Nicolas Ortonne,Olivier Tournilhac,Philippe Gaulard,Richard Delarue,Romain Pelletier,Virginie Fataccioli

Transferring a Quantitative Molecular Diagnostic Test to Multiple Real-Time Quantitative PCR Platforms

Claudia Gürtler,Heike Steinh？user,Kornelia Schlombs,Mark Laible,Shujin Liu,Ugur Sahin,Wolfgang Schwabe,Xingmin Li