【Objective】 To discover the effects of biochar on soil bacterial community diversity, abundance and structure of different rice varieties, and provide a scientific basis for the application of biochar in paddy field.【Method】 Experiment of split-split plot was designed in the late rice field of Fluvo-aquic soil type in Taishan Town of Jiangmen County, Guangdong Province in 2019, treatments were designed with no biochar application(CK), biochar application of 3.5 t/hm2 (Tr1) and 7 t/hm2 (Tr2) as t...

Agricultural Science  Soil Science  Agronomy 

Doi: 10.7671/j.issn.1001-411X.202108022

【Objective】 To explore the effects of drought stress on maize tassel development characteristics and yield, and provide a theoretical basis for drought resistance and yield protection of maize planting in northeast China.【Method】 In the pot experiment, original soil treatment and adding soil conditioner treatment were set up. Different drought stress degrees of normal water supply (CK), light drought stress, moderate drought stress, and severe drought stress were conducted from the big trumpet s...

Agricultural Science  Plant Science  Agronomy 

Doi: 10.7671/j.issn.1001-411X.202107025

【Objective】 The acquisition of high quality disease-resistant rice maintainers is the premise of improving the quality and resistance of sterile lines. The molecular marker-assisted selection (MAS) can be used for oriented breeding and accelarating the breeding process of elite rice maintainers.【Method】 ‘Hengfeng B’ and ‘Guang 8B’ were used as recurrent parents, and ‘B39’ carrying fragrance gene Badh2and rice blast resistance gene Pita was used as donor parent. The successive backcross breeding ...

Agricultural Science  Molecular Biology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202109021

【Objective】 The purpose of this study was to investigate the effects of dietary supplementation of different conjugated linoleic acid (CLA) isomers on body fat, energy metabolism and gut microbiota in mice.【Method】 Mice were fed with normal diet (control group), normal diet supplemented with 2% cis-9, trans-11 conjugated linoleic acid (c9, t11-CLA) and trans-10, cis-12 conjugated linoleic acid (t10, c12-CLA) for 12 weeks respectively. The changes of fat content, energy metabolism and gut microbi...

Agricultural Science  Zoology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202110016

【Objective】 In order to meet the field operation requirements, the UAV needs not only a rotor with the function of flexible take-off and landing, but also fixed wings with the characteristics of long endurance. A new agricultural plant protection UAV with X-shaped rotor and fixed wing hybrid structure as well as tilting function was designed.【Method】 According to the load dynamic requirements of farmland spraying operation, the airfoil and parameters of the lifting device of UAV were designed. T...

Agricultural Science  Agricultural Engineering  Agronomy 

Doi:10.7671/j.issn.1001-411X.202111007

【Objective】 The goal was to in depth study the pruning process characteristics and cutting mechanism of fruit branches, and provide a reference for optimizing the core cutting components of pruning machines and improving the cutting performance of electric pruning machines.【Method】 Taking the branch of ‘Shixia’variety of longan as the test object, ANSYS/LS-DYNA was used to perform finite element simulation of the branch cutting process. We built a branch cutting test platform for cutting experim...

Agricultural Science  Agricultural Engineering  Agronomy 

Doi: 10.7671/j.issn.1001-411X.202111010

【Objective】 An expert system for diagnosing citrus diseases and pests based on fault tree analysis was developed to solve the problems of insufficient popularization of professional plant protection knowledge and lack of efficient diagnosis methods in the prevention and control of citrus diseases and pests.【Method】 First, fault tree analysis method was used to calculate the occurrence probability of diseases and pests and establish a knowledge base of diseases and pests. Secondly, based on the k...

Agricultural Science  Technology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202110037

【Objective】 To clarify the species of stem nematode from diseased sample of Codonopsis pilosula in Gansu Province. 【Method】 Species identification of stem nematode was carried out by combing morphological analysis, phylogenetic analyses of ITS-rDNA and 28S-rDNA sequences, PCR with species-specific primers as well as PCR-ITS-RFLP.【Result】 The morphological characteristics of the stem nematode population from C. pilosula in Gansu was similar to those of Ditylenchus arachis. The ranges of measureme...

Agricultural Science  Parasitology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202111034

【Objective】 Mulberry bacterial wilt is a seriously harmful bacterial disease caused by Ralstonia solanacearum. Therefore, it is of great significance to establish a rapid and sensitive detection method for R. solanacearum to effectively control mulberry bacterial wilt. 【Method】 Pectate lyase gene of R. solanacearum was used as the target. Based on the primer design principle of isothermal multiple self-matching-initiated amplification (IMSA), combining the loop-mediated isothermal amplification ...

Agricultural Science  Cell Biology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202203030

【Objective】 In order to study the colonization rule of Bacillus subtilis biocontrol strain SEM-9 in the rhizosphere of crops and its influence on the microbial diversity of rhizosphere soil. 【Method】 The strain SEM-9 was labeled with green fluorescent protein by natural transformation method, and the colonization in rhizosphere soil, on root surface and in root tissue were observed by inverted fluorescence microscope. The changes of microbial diversity in rhizosphere soil treated with the strain...

Agricultural Science  Soil Science  Agronomy 

Doi:10.7671/j.issn.1001-411X.202107008