We describe a planar MEMS silicon structure to record ion channel currents in biological cells. The conventional method of performing an electrophysiological experiment, 'patch-clamping', employs a glass micropipette. The micropipette tip is a source of thermal noise because of its inherent, tapered, conical structure, giving rise to a large pipette resistance. This pipette resistance, when coupled with the biological cell capacitance, limits the available bandwidth of single ion channel recordi...

Taxonomy  Microbiology  Biochemistry  Biodiversity  Developmental Biology  Bioengineering  Bioinformatics  Environmental Sciences  Agricultural Science  Computational Biology  Genetics  Cell Biology  Food Science & Technology  Parasitology  Synthetic Biology 

Doi:10.1016/j.sse.2004.05.072

Computer simulations of realistic ion channel structures have always been challenging and a subject of rigorous study. Simulations based on continuum electrostatics have proven to be computationally cheap and reasonably accurate in predicting a channel's behavior. In this paper we discuss the use of a device simulator, SILVACO, to build a solid-state model for KcsA channel and study its steady-state response. SILVACO is a well-established program, typically used by electrical engineers to simula...

Biological Chemistry  Photochemistry  Taxonomy  Medicinal Chemistry  Ecosystem Science  Developmental Biology  Computational Robotics  Complex network models  Artificial Intelligence  Genetics  Theoretical Chemistry  Analytical Chemistry  Neuroscience  Biological Materials  Nanometer Materials  Green Chemistry  Mathematical Biology  Molecular Biology  Microbiology  Biochemistry  Biodiversity  Bioengineering  Bioinformatics  Biophysics  Biotechnology  Functional Materials  Computational Biology  Synthetic Biology 

Doi:10.1016/j.cmpb.2006.08.007

Infections from parasitic nematodes (or roundworms) contribute to a significant disease burden and productivity losses for humans and livestock. The limited number of anthelmintics (or antinematode drugs) available today to treat these infections are rapidly losing their efficacy as multidrug resistance in parasites becomes a global health challenge. We propose an engineering approach to discover an anthelmintic drug combination that is more potent at killing wild-type Caenorhabditis elegans wor...

Neuroscience  Marine Biology  Toxicology  Taxonomy  Microbiology  Ecosystem Science  Biodiversity  Developmental Biology  Bioengineering  Environmental Sciences  Animal Behavior  Agricultural Science  Biotechnology  Computational Biology  Food Science & Technology  Parasitology  Plant Science  Synthetic Biology 

Doi:10.1126/sciadv.aao1254

In this paper, we explore the use of microfluidic paper-based analytical devices (？PADs) to study the behavior of Caenorhabditis elegans. We show how these devices can be fabricated on paper and plastic substrates, as well as how to load, visualize, and transfer single and multiple nematodes. We also demonstrate the use of anthelmintic drug, levamisole, to perform chemical testing on C. elegans. Furthermore, we provide a custom program that is able to recognize individual worms on the ？PADs in r...

Animal Behavior  Computational Biology  Microbiology  Cell Biology  Parasitology  Plant Science  Soil Science  Developmental Biology  Bioengineering  Environmental Sciences  Biophysics 

Doi:10.1063/1.5005829

Q fever which is seen in most countries throughout the world, is a zoonotic disease caused by Coxiella burnetii a bacteria of Gram negative, obligate intracellular. The purpose of this study was to search the seroprevalence of C.burnetii and Q fever in risk groups in Kahramanmara？ city, Turkey. A total of 40 voluntary individuals (34 male, 6 female; age range, 20-60years) including veterinarians, cattle-dealers, and butchers working in Kahramanmara？ city were included in the study. C.burnetii ph...

Microbiology 

Doi:10.25279/sak.506991

The mitochondrion is a natural robot (cell) with a length of about one micron. Between organisms (plant, animal or human) with its organs and the atomic and molecular components are positioned based cells, which are building all the necessary organs body. Years ago, medical specialists who are studying organ disease have considered that is caused by impaired organ or related to other organs. For example, it was considered that all nerve diseases have started from stress due to disorder of the hy...

Neuroscience  Public Health  Biotechnology  Molecular Biology  Genetics  Microbiology  Cell Biology  Biochemistry  Bioengineering  Medical Genetics 

Doi:10.3844/ajeassp.2016.991.1002

Mammalian esophagus exhibits a remarkable change in epithelial structure during the tran- sition from embryo to adult. However, the molecular mechanisms of esophageal epithelial development are not well understood. Zebrafish (Danio rerio), a common model organism for vertebrate development and gene function, has not previously been characterized as a model system for esophageal epithelial development. In this study, we characterized a piece of non-keratinized stratified squamous epithelium ...

Neuroscience  Microbiology 

Doi:10.1371/journal.pone.0143878

Grewia asiatica L., is a species native to south Asia from Pakistan, east to Cambodia, cultivated primarily for its edible fruit and well-reputed for its diverse medicinal uses. Fruits are a rich source of nutrients such as proteins, amino acids, vitamins, and minerals and contain various bioactive compounds, like anthocyanins, tannins, phenolics and flavonoids. Different parts of this plant possess different pharmacological properties. Leaves have antimicrobial, anticancer, antiplatelet and ant...

Biological Chemistry  Cardiology  Toxicology  Photochemistry  Taxonomy  Medicinal Chemistry  Environmental Sciences  Agricultural Science  Genetics  Food Science & Technology  Nutrition  Analytical Chemistry  Biological Materials  Environmental Chemistry  Green Chemistry  Pharmacology  Public Health  Organic Chemistry  Evidence Based Medicine  Gastroenterology & Hepatology  Microbiology  Global Health  Biochemistry  Biodiversity  Biophysics  Biotechnology  Translational Medicine  Respiratory Medicine 

Doi:10.3390/molecules18032663

The aim of this study was to investigate the effects of the mushroom extract of Coriolus versicolor on drug resistant strain of S. typhimurium experimental results revealed that hot water ethanol extract and Tris- HCl ethanol extract of C. versicolor were not only effective against drug resistant strain of S.typhimurium (MTCC 3214) but also against other pathogenic microorganisms such as K. pneumoniae, S. typhi, E. coli, S. pyogenes and Aspergillus niger. The extracts exerted their effects on th...

Streptomyces sp. VRY-1 was screened for its bioactive potentials against various pathogenic microorganisms and was found to possess antibacterial activity against Salmonella typhimurium (drug resistant strain of bacteria) and various other pathogenic bacteria and fungi. Optimization of cultural conditions for production of antibiotic showed that maximum antibiotic production occurred on 10th day in stationary cultural, 28？？ C, 8.0 pH, liver extract, 1.5%(w/v) glucose. The bioactive compound was...