Cold atmospheric plasma (CAP) has received great attention due to its noteworthy ability, and has also been widely studied over few decades in physics, biology and medicine. The purpose of this study is to evaluate the cold atmospheric pressure plasma effects on the proliferation of breast cancer cells. MDAMB-231 was used for this experiment. MDA-MB-231 cells were cultured in 24-well plate and treated with non-thermal plasma. The viability of the cancer cells was determined by MTT assay. Differe...

Plasma  Cell Biology 

Doi:10.29252/pbr.3.3.12

We report a nematode electrotactic-response valve (NERV) to control the locomotion of Caenorhabditis elegans (C. elegans) in microfluidic devices. This nonmechanical, unidirectional valve is based on creating a confined region of lateral electric field that is switchable and reversible. We observed that C. elegans do not prefer to pass through this region if the field lines are incident to its forward movement. Upon reaching the boundary of the NERV, the incident worms partially penetrate the fi...

Animal Behavior  Zoology  Mathematical Biology  Veterinary Medicine  Cell Biology  Parasitology  Developmental Biology  Evolutionary Studies  Bioengineering  Bioinformatics  Environmental Sciences  Biophysics 

Doi:10.1063/1.3570629

We describe a planar MEMS silicon structure to record ion channel currents in biological cells. The conventional method of performing an electrophysiological experiment, 'patch-clamping', employs a glass micropipette. The micropipette tip is a source of thermal noise because of its inherent, tapered, conical structure, giving rise to a large pipette resistance. This pipette resistance, when coupled with the biological cell capacitance, limits the available bandwidth of single ion channel recordi...

Taxonomy  Microbiology  Biochemistry  Biodiversity  Developmental Biology  Bioengineering  Bioinformatics  Environmental Sciences  Agricultural Science  Computational Biology  Genetics  Cell Biology  Food Science & Technology  Parasitology  Synthetic Biology 

Doi:10.1016/j.sse.2004.05.072

In this paper, we explore the use of microfluidic paper-based analytical devices (？PADs) to study the behavior of Caenorhabditis elegans. We show how these devices can be fabricated on paper and plastic substrates, as well as how to load, visualize, and transfer single and multiple nematodes. We also demonstrate the use of anthelmintic drug, levamisole, to perform chemical testing on C. elegans. Furthermore, we provide a custom program that is able to recognize individual worms on the ？PADs in r...

Animal Behavior  Computational Biology  Microbiology  Cell Biology  Parasitology  Plant Science  Soil Science  Developmental Biology  Bioengineering  Environmental Sciences  Biophysics 

Doi:10.1063/1.5005829

The complex biomechanics and morphology of the femur proximal epiphysis are presented. This specific region in human femur is characterized by a high flexibility compared to that of other primates, since evolved lighter and longer due to the human verticalposition and more balanced loading. The nature and fine morphology of the femur head and its structural behavior have been investigated. Isotropic and orthotropic trabecular structures, which are not present in other primates, have been associa...

Biological Materials  Public Health  Biological Chemistry  Anatomy & Physiology  Dentistry  Global Health  Biochemistry  Bioengineering  Biophysics  Biotechnology  Biological Engineering  Computational Biology  Cell Biology  Orthopedics  Synthetic Biology  Internal Medicine  Surgery & Surgical Specialties 

Doi:10.3844/ajeassp.2016.1213.1221

The four basic elements of life are: Oxygen, hydrogen, nitrogen and phosphorus. These four elements are found in abundance in both the human body and in animals. There are other elements that compose the human body, but the four we've highlighted participate in all life processes. Besides, these four elements make up ATP chains (molecule), which governs and controls the body entirely energy processes and physiological and pathological processes of the human body. Oxygen is the pivot, which produ...

Biological Materials  Nanometer Materials  Genetic Engineering  Industrial Engineering  Biological Chemistry  High Polymer Chemistry  Inorganic Nonmetallic Materials  Civil Engineering  Geriatrics  Composite Material  Global Health  Structural Material  Biochemistry  Bioengineering  Fundamentals of Material Science  Biophysics  Biological Engineering  Biotechnology  Functional Materials  Chemical Engineering & Technology  Genetics  Cell Biology  Material Experiment 

Doi:10.3844/ajeassp.2016.1189.1197

The mitochondrion is a natural robot (cell) with a length of about one micron. Between organisms (plant, animal or human) with its organs and the atomic and molecular components are positioned based cells, which are building all the necessary organs body. Years ago, medical specialists who are studying organ disease have considered that is caused by impaired organ or related to other organs. For example, it was considered that all nerve diseases have started from stress due to disorder of the hy...

Neuroscience  Public Health  Biotechnology  Molecular Biology  Genetics  Microbiology  Cell Biology  Biochemistry  Bioengineering  Medical Genetics 

Doi:10.3844/ajeassp.2016.991.1002

Clinical evidence has shown that physical exercise during pregnancy may alter brain devel- opment and improve cognitive function of offspring. However, the mechanisms through which maternal exercise might promote such effects are not well understood. The present study examined levels of brain-derived neurotrophic factor (BDNF) and absolute cell num- bers in the hippocampal formation and cerebral cortex of rat pups born from mothers exer- cised during pregnancy. Additionally, we evaluated the cog...

Cell Biology 

Doi:10.1371/journal.pone.0147200

Knowledge of polyomavirus BK (BKV) genomic diversity has greatly expanded. The implications of BKV DNA sequence variation for the performance of molecular diagnostic assays is not well studied. We analyzed 184 publically available VP-1 sequences encompassing the BKV genomic region targeted by an in-house quantitative hydrolysis probe-based PCR assay. A perfect match with the PCR primers and probe was seen in 81 sequences. One Dun and 13 variant prototype oligonucleotides were synthesized as arti...

Doi:10.1128/JCM.01230-11

Alemtuzumab was used as an induction agent in 205 renal transplant recipients undergoing 207 living donor renal transplants. All donor kidneys were recovered laparoscopically. Postoperatively, patients were treated with tacrolimus monotherapy, and immunosuppression was weaned when possible. Forty-seven recipients of living donor renal transplants prior to the induction era who received conventional triple drug immunosuppression without antibody induction served as historic controls. The mean fol...

Doi:10.1111/j.1600-6143.2006.01495.x