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OALib Journal期刊
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Biosynthetic origin of the isoprene units in chromenes of Piper aduncum (Piperaceae)

DOI: 10.1590/S0103-50532007000800008

Keywords: piper aduncum, chromenes, [1-13c]-d-glucose, mevalonate pathway, 2-c-methyl-d-erythritol-4-phosphate pathway.

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Abstract:

metabolic studies involving the incorporation of [1-13c]-d-glucose into intact leaves of piper aduncum (piperaceae) have indicated that both the mevalonate (mva) and the pyruvate-triose (mep) non-mevalonate pathways are implicated in the biosynthesis of isoprene moieties present in methyl 2,2-dimethyl-2h-1-chromene-6-carboxylate (1) and methyl 2,2-dimethyl-8-(3'-methyl-2'-butenyl)-2h-1-chromene-6-carboxylate (2). the pattern of incorporation of label from [1-13c]-d-glucose into these chromenes was determined by quantitative 13c nmr spectroscopy. the results confirmed that biosynthetic compartment of 1 and 2 could either be the plastid and/or the cytosol or, possibly, an additional compartment such as the plastid inter-membrane space.

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