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Identificación molecular del hongo Colletotrichum spp., aislado de banano (Musa spp) de la altura en la zona de Turrialba y determinación de su sensibilidad a fungicidas poscosecha

Keywords: anthracnose, pcr, postharvest, thiabendazole, imazalil, miclobutanil.

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Abstract:

anthracnose is one of the most serious banana post-harvest diseases. a total of 25 colletotrichum isolates were obtained from banana fruits grown in 5 areas in turrialba county, costa rica; 5 isolates per area. isolates were characterized on the basis of polymerase chain reaction (pcr) using species-specific primers of the its region and ?-tubulin, gene, and their in vitro sensitivities to the fungicides thiabendazole, imazalil and myclobutanil at 0.1, 1.0, 10.0 y 100.0 μg.ml-1. all isolates were identified as colletotrichum gloeosporioides and were sensitive to thiabendazole, whereas most isolates showed a marked reduction in sensitivity to imazalil and myclobutanil.

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