This work presents the pathology
description, isolation and identification of canine herpesvirus (CHV-1) in
Mexico, a virus that causes a generalized hemorrhagic infection in puppies from
the canidae family. Methods: Isolates were obtained from puppies that died
within the first four weeks of life and had lesions consistent with canine
herpesvirus. Results: The main gross lesions were petechial and ecchymotic
hemorrhages in kidneys, liver and lungs; proliferative interstitial nephritis;
multifocal necrosis in liver and kidneys; and encephalitis with intranuclear
inclusion bodies. Herpesvirus was confirmed through direct immunofluorescence,
electron microscopy and polymerase chain reaction for DNA polymerase and
glycoprotein B genes. Discussion: Eight strains were isolated and identified as
canine herpesvirus corresponding to three of the working cases with gross and
microscopic lesions very similar to those described in the literature; then,
isolates were confirmed by PCR gene amplification, positive reactions on
immunofluorescence and observations from electron microscopy. This work
represents the first report of this disease, including gross and histological
lesions, and confirmation by isolation and identification of the canine
herpesvirus in Mexico.
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