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Pathology Isolation and Identification of Canine Herpesvirus (CHV-1) in Mexico

DOI: 10.4236/ojpathology.2016.63014, PP. 111-121

Keywords: Pathology of Puppies, Canine Herpesvirus, PCR, Electron Microscopy

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Abstract:

This work presents the pathology description, isolation and identification of canine herpesvirus (CHV-1) in Mexico, a virus that causes a generalized hemorrhagic infection in puppies from the canidae family. Methods: Isolates were obtained from puppies that died within the first four weeks of life and had lesions consistent with canine herpesvirus. Results: The main gross lesions were petechial and ecchymotic hemorrhages in kidneys, liver and lungs; proliferative interstitial nephritis; multifocal necrosis in liver and kidneys; and encephalitis with intranuclear inclusion bodies. Herpesvirus was confirmed through direct immunofluorescence, electron microscopy and polymerase chain reaction for DNA polymerase and glycoprotein B genes. Discussion: Eight strains were isolated and identified as canine herpesvirus corresponding to three of the working cases with gross and microscopic lesions very similar to those described in the literature; then, isolates were confirmed by PCR gene amplification, positive reactions on immunofluorescence and observations from electron microscopy. This work represents the first report of this disease, including gross and histological lesions, and confirmation by isolation and identification of the canine herpesvirus in Mexico.

References

[1]  Lacheretz, A. and Cognard, S. (1998) Epidemiology and Serological Diagnosis of 10 Canine Herpesvirus. RevMédVét, 143, 1477-1488.
[2]  Reading, M.J. and Field, H.J. (1998) A Serological Study of Canine Herpes Virus-1 Infection in the English Dog Population. Archives of Virology, 143, 1477-1488.
http://dx.doi.org/10.1007/s007050050391
[3]  Rijsewijk, F.A.M., Luiten, E.J., Daus, F.J., Van der Heijden, R.W. and Van Oirschot, J. (1999) Prevalence of Antibodies against Canine Herpesvirus 1 in Dogs in the Netherlands in 1997-1998. Veterinary Microbiology, 65, 1-7.
http://dx.doi.org/10.1016/S0378-1135(98)00285-5
[4]  Ronsse, V., Verstegen, J., Onclin, K., Guiot, A.L., Aeberle, C. and Nauwynck, H.J. (2002) Seroprevalence of Canine Herpesvirus-1 in the Belgian Dog Population in 1997-1998. Reproduction Domestic Animals, 37, 299-304.
http://dx.doi.org/10.1046/j.1439-0531.2002.00363.x
[5]  Carmichael, L.E. and Medic, B.L. (1978) Small-Plaque Variant of Canine Herpesvirus with Reduced Pathogenicity for Newborn Pups. Infection and Immunity, 20, 108-114.
[6]  Ledbetter, E.C., Dubovi, E.J., Sung, G., Maggs, D. and Bicalho, R. (2009) Experimental Primary Ocular Canine Herpesvirus-1 Infection in Adult Dogs. American Journal of Veterinary Research, 70, 513-521.
http://dx.doi.org/10.2460/ajvr.70.4.513
[7]  Percy, D.H., Olander, H.J. and Carmichael, L.E. (1968) Encephalitis in the Newborn Pup Due to a Canine Herpesvirus. Veterinary Pathology, 5, 135-145.
[8]  Strandberg, J.D. and Aurelian, L. (1969) Replication of Canine Herpesvirus. II. Virus Development and Release in Infected Dog Kidney Cells. Journal of Virology, 4, 480-489.
[9]  Takumi, A., Kusanagi, K., Tuchiya, K., Xuan, X. and Azetaka, M. (1989) Serodiagnosis of Canine Herpesvirus Infection-Development of an Enzime-Linked Immunosorbent Assay and Its Comparison with Two Improved Methods of Serum Neutralization Test. Japan Journal Veterinary Science, 52, 241-250.
http://dx.doi.org/10.1292/jvms1939.52.241
[10]  VanDevanter, D.R., Warrener, P., Bennett, L., Schultz, E.R., Coulter, S., Garber, R.L. and Rose, T.M. (1996) Detection and Analysis of Diverse Herpes Viral Species by Consensus Primer PCR. Journal of Clinical Microbiology, 34, 1666-1671.
[11]  Burr, P.D., Campbell, M.E.M., Nicolson, L. and Onions, D.E. (1996) Detection of Canine Herpesvirus 1 in a Wide Range of Tissues Using the Polymerase Chain Reaction. Veterinary Microbiology, 53, 227-237.
http://dx.doi.org/10.1016/S0378-1135(96)01227-8
[12]  Carmichael, L.E., Squire, R.A. and Krook, L. (1965) Clinical and Pathologic Features of a Fatal Viral Disease of Newborn Pups. American Journal of Veterinary Research, 26, 803-814.
[13]  Love, D.N. and Huxtable, C.R. (1976) Naturally-Occurring Neonatal Canine Herpesvirus Infection. The Veterinary Record, 99, 501-503.
http://dx.doi.org/10.1136/vr.99.25-26.501
[14]  Poulet, H., Guigal, P.M., Soulier, M., Leroy, V., Fayert, G., Minke, J. and Chappuis, M. (2001) Protection of Puppies against Canine Herpesvirus by Vaccination of the Dams. The Veterinary Record, 148, 691-695.
http://dx.doi.org/10.1136/vr.148.22.691
[15]  Wright, N.G. and Cornwell, H.J. (1968) Experimental Herpes virus Infection in Young Puppies. Research in Veterinary Science, 9, 295-299.
[16]  McGavin, M.D. and Zachary, J.D. (2007) Pathological Basis of Veterinary Disease. 4th Edition, Mosby-Elsevier, St Louis Missouri.
[17]  Decaro, N., Amorisco, F., Desario, C., Lorusso, E., Camero, M., Bellacicco, A.L., Sciarretta, R., Lucente, M.S., Martella, V. and Buonavoglia, C. (2010) Development and Validation of a Real-Time PCR Assay for Specific and Sensitive Detection of Canid Herpesvirus 1. Journal of Virological Methods, 169, 176-180.
http://dx.doi.org/10.1016/j.jviromet.2010.07.021
[18]  Greene, C.E. (2012) Canine Herpevirus Infection. 4th Edition, Saunders, New York.
[19]  Maurer, U.E., Zeev-Ben-Mordehai, T., Pandurangan, A., Cairns, T., Hannah, B., Whitbeck, J.C., Eisenberg, R.J., Cohen, G.H., Topf, M. and Huiskonen, J.T. (2009) The Structure of Herpesvirus Fusion Glycoprotein B-Bilayer Complex Reveals the Protein-Membrane and Lateral Protein-Protein Interaction. Cell Structure, 21, 1396-1405.
http://dx.doi.org/10.1016/j.str.2013.05.018
[20]  Spear, P.G. (2004) Herpes Simplex Virus: Receptors and Ligands for Cell Entry. Cellular Microbiology, 6, 401-410.
http://dx.doi.org/10.1111/j.1462-5822.2004.00389.x
[21]  Roizmann, B., Desrosiers, R.C., Fleckenstein, B., Lopez, C., Minson, A.C. and Studdert, M.J. (1992) The Family Herpesviridae: An Update. The Herpesvirus Study Group of the International Committee on Taxonomy of Viruses. Archives of Virology, 123, 425-449.
http://dx.doi.org/10.1007/BF01317276
[22]  Wagner, M., Ruzsics, Z. and Koszinowski, U.H. (2002) Herpesvirus Genetics Has Come of Age. Trends in Microbiology, 10, 318-324.
http://dx.doi.org/10.1016/S0966-842X(02)02394-6

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