oalib
Search Results: 1 - 10 of 100 matches for " "
All listed articles are free for downloading (OA Articles)
Page 1 /100
Display every page Item
Canine Herpesvirus Seroprevalence and Associated Factors in Dogs of Mexico  [PDF]
Edgar Guillermo Valdivia Lara, Jesica Ileana ángeles Solis, Cesar Cuenca Verde, Juan Antonio Montaraz Crespo, Laura Cobos Marín, Juan Carlos Del Río García, Guillermo Valdivia Anda
Open Journal of Veterinary Medicine (OJVM) , 2016, DOI: 10.4236/ojvm.2016.610019
Abstract:
Canine herpesvirus (CHV-1) causes disease associated with high mortality in infect-ed puppies, which represents large financial losses for dog breeders. Since CHV-1 at the time of the study he had not been reported in Mexico, the main objective of this study was to determine the prevalence of antibodies against CHV-1 in canine kennels in the metropolitan area of Mexico City. A commercial enzyme-linked immuno-sorbent assay (ELISA) was used, and the results were compared to those of a viral neutralization test. The ELISA kit uses the complete viral particle as the antigen. The plaque reduction neutralization test was combined with the immunoperoxidase technique because of the low cytopathic effect of CHV-1. Neutralizing antibodies were also detected in 20 randomly selected samples. The prevalence of CHV-1 with ELISA was 87%. The concordance between ELISA and serum neutralization (SN) was 0.1129, the sensitivity of the ELISA against SN was 1.0 (100%), the positive predic-tive value was 0.39 (39%), and the negative predictive value was 1 (100%). These results show that ELISA is useful for monitoring the dog population for CHV-1; a positive test result requires confirmation with an SN test, and a negative ELISA result indicates a high probability of being SN-negative. The only variables that were sta-tistically associated with CHV-1 prevalence were breed and kennel. A statistically significant relationship between the degree of ELISA and SN titer was obtained, with a confidence level of 95%. None of the clinical presentation factors was statistically significant. These results suggest that most of the canine population studied in Mex-ico is in a herpesvirus latency state.
Investigation of DNA Sequences Related to Latency-Associated Transcripts in the Genome of Canine Herpesvirus Type 1 (CHV-1) by Means of Bioinformatics Tools  [PDF]
Ortiz M. A. Hernández, Verde C. Cuenca, Lara E. G. Valdivia, Anda G. Valdivia
Open Journal of Veterinary Medicine (OJVM) , 2019, DOI: 10.4236/ojvm.2019.910013
Abstract: A characteristic common to herpesviruses is the ability to establish a latent infection in the hosts, a transcriptionally active region has detected during latency as well as a set of RNA that are known as Latency Associated Transcripts (LATs), their functions have been clarified in recent work. The present work was carried using different bioinformatics method in order to determine if Herpesvirus Canine 1 (CHV-1) has a region associated with latency. Our result was the selection of nine sequences candidate of micro RNA (miRNA) (MIREval 2.0 software), and 26 miRNA (miRNAFold v.1.0 software), of them, were selected 14 with real precursors of miRNA, two were found between the RL2 and RS1 genes, one in the RL2 gene and 11 in the RS1 gene. The results showed that the similarities of these regions are very low among the herpesviruses analyzed, so it was not possible to deduce the presence of the LAT gene in canine herpesvirus type 1 with bioinformatics. On the other hand, the comparison showed that the miRNA predicted: chv1-mir-mirnafold-8 has similarity with the ebv-mir-BART7-3p of Epstein-Barr Virus (EBV), in this way, the microRNAs predicted by means of bioinformatic programs met the theoretical requirements of these molecules, however at not having a degree of preservation in other herpesviruses, the expression by CHV-1 in latency cannot be confirmed and it is necessary to identify through experimental tests.
A Serologic Investigation of Canine Herpesvirus Type 1 Infection in Kangal Dogs
Abuzer Acar,Sibel Gur,Ismet Dogan,Yilmaz Akca
Journal of Animal and Veterinary Advances , 2012,
Abstract: Canine Herpesvirus-1 (CHV-1) is an Alphaherpesvirus, the infection was detected throughout the world in dog population. In this study, 110 blood serum samples obtained from clinically healthy adult Kangal dogs, which are unvaccinated for CHV-1 in 4 private Kangal breeding kennels at Ankara province. The samples was controlled using indirect ELISA, as result of the test, out of 110 dog sera, 79 (71.8%) was found to be seropositive for CHV1. Positivity was found to be in all of the studied farms among 42.8 and 86.6% proportions. The important differences were not detected between male (21/32, 65.6%) and female (58/78, 74.3%) dogs as statistically. According to kennel records, no clinical findings related to the CHV1 infection were reported in any of the animals before, despite very high infection rates.
Stimulation of Phagocytosis and Production of Antibodies against Canine Herpesvirus Type 1 by Pidotimod (AdimodTM)  [PDF]
Edgar G. Valdivia Lara, Andrés ángeles Aldana, Jesica I. ángeles Solis, Patricia Miranda Castro, Juan C. Del Río García, Guillermo Valdivia Anda
Open Journal of Veterinary Medicine (OJVM) , 2017, DOI: 10.4236/ojvm.2017.74005
Abstract: Neutrophils are the most important circulating phagocytes. Circulating mono-cytes and precursors of tissue macrophages also have the ability to phagocytize. Pidotimod (ADIMODTM) exerts immunostimulatory and immunoregulatory effects through the stimulation and regulation of cellular immune responses by lymphocytes Canine herpesvirus (CHV) mainly affect puppies between the first and second weeks of age, causing high morbidity in the litter. To date, there is only one commercial vaccine in Europe to prevent disease. In this work, inactivated CHV cultures were inoculated in rabbits, adsorbed and not adsorbed to chitosan nanoparticles. Phagocytosis in the presence or absence of specific antibodies was measured. Response of virus neutralizing antibodies was also evaluated. AdimodTM enhanced the nonspecific and specific phagocytotic response. The association of the virus to the nanoparticles increased the phagocytic ability of blood cells; however, AdimodTM alone had a greater effect on phagocytic activity and generated a stronger immune response that corresponded to the increased phagocytosis (p < 0.05). Moreover, the level of neutralizing antibodies was higher and increased more rapidly when AdimodTM was used.
THE ROLE OF REPRESENTATIVES IN OPHTHALMIC PATHOLOGY HERPESVIRUS
Smelyanskaya MV,,Peremot SD,,Martynov AV,,Volyansky AY
Annals of Mechnikov's Institute , 2012,
Abstract: The goal was to establish a place of herpesviruses (HSV1, HSV2, HHV6, SMV, VZV, EBV) in the etiological structure of the eye’s diseases. The study involved 35 patients diagnosed with recurrent keratitis (15 patients) and viral uveitis (n = 20). Was carried out virological examination, including a study using fluorescent antibody scrapings from the cornea and conjunctiva for the presence of herpesvirus antigen in leukocytes and determination of the blood antigens of viruses with the calculation of the fluorescence index (FI). Set of different herpesvirus persistence in leukocytes of blood in 95% of patients with viral uveitis and 32% of patients with keratitis. In the each second patient with viral uveitis (10) detected association of three or more herpesvirus antigens in blood leukocytes. Most often these associations are found antigens of viruses EBV, SMV, and HHV6.
Detección de virus herpes canino tipo 1 en Chile Canine herpesvirus-1 detection in Chile  [cached]
C NAVARRO,M CELEDON,J PIZARRO
Archivos de medicina veterinaria , 2003,
Abstract: RESUMEN Este trabajo informa la detección del virus herpes canino tipo 1 (VHC-1) en nuestro país, confirmando la fuerte sospecha clínica de su existencia. Se logró obtener un aislado viral, denominado RP5, a partir de casos clínicos diagnosticados como enfermedad hemorrágica de los cachorros. Este aislado inoculado en monocapas celulares produce el típico efecto citopático de miembros de la subfamilia alphaherpesvirinae, familia Herpesviridae: lisis celular a tiempos cortos. Este aislado manifiesta este efecto tanto en cultivos primarios de pulmón y ri ón canino, como también en la línea celular MDCK. Este efecto se pierde al preincubar el inóculo con solventes orgánicos, lo cual indica presencia de envoltura lipoproteica, lo cual descartaría la presencia de VAC-1 en este aislado. Como segunda confirmación de la presencia de VHC-1 en los cultivos celulares inoculados se procedió a realizar una prueba de inmunofluorescencia directa, utilizando un anticuerpo monoclonal anti VHC-1, conjugado con isotiocianato de fluoresceína (VMRD, Inc.), cuyos resultados se alan inequívocamente la presencia del virus. Determinar la presencia del virus es de gran importancia, pues al aislar y caracterizar el virus se podrá contar con un instrumento imprescindible para realizar futuras investigaciones. Por ejemplo, evaluar la magnitud de la infección en la especie canina, conocer de la repercusión de la infección por este virus en planteles reproductores, como también enfrentar el desafío del dise o de métodos diagnóstico de laboratorio mediante técnicas que involucran la inmunofluorescencia, el enzimoinmunoanálisis (ELISA) y la reacción en cadena de la polimerasa (PCR), y también elaborar posibles estrategias mediante la utilización de una eventual vacuna. SUMMARY This work reports the detection of canine herpesvirus type 1 (CHV-1) in Chile, confirming the strong clinical suspicion of its existence. A viral isolate, RP5, was obtained from diagnosed clinical cases such as hemorrhagic diseases of puppies. This viral isolate was inoculated in cell monolayers and produced the typical cytopathic effect of members of the Alphaherpesvirinae subfamily, Herpesviridae family: cellular lysis in a short period of time. This viral isolate demostrated its effect in primary cultures of canine lung and canine kidney, and also in the MDCK cell line. This effect was lost due to prior incubation of the inoculate with an organic solvent, therefore the adenovirus presence was ruled out. As a specific confirmation of VHC-1 presence, a direct fluorescence test in inoculated cell cultures was car
Prevalence of Canine Heartworm in Dogs from Monterrey, Mexico
J.A. Salinas-Melendez,E. Pope-Gonzalez,B.V. Tamez-Hernandez,J.J. Zarate-Ramos,G. Davalos-Aranda,R. Avalos-Ramirez,M.A. Cantu-Martinez,V.M. Riojas-Valdes
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.756.759
Abstract: Canine dirofilariosis is a vector-borne disease caused by Dirofilaria immitis and causes severe clinical signs. A high prevalence has been reported in the USA and other countries while in Mexico it has been lower. In the present study 391 dogs were sampled at random at the city of Monterrey, Mexico and were tested by the ELISA method using a canine commercial kit. Only 28 animals resulted positive (7%) and included different dog breeds and mixed blood animals. According to sex, 18 were males and 10 females while the prevalence was higher in animals with short hair. According to exposition to pest control and place of stay, animals that have been exposed to insecticides and were kept outside the house had a higher prevalence of D. immitis. These results are in agreement with previous studies that have also performed random sampling in the same city.
A review of the pathology and treatment of canine respiratory infections
Vieson MD, Pi eyro P, LeRoith T
Veterinary Medicine: Research and Reports , 2012, DOI: http://dx.doi.org/10.2147/VMRR.S25021
Abstract: review of the pathology and treatment of canine respiratory infections Review (2363) Total Article Views Authors: Vieson MD, Pi eyro P, LeRoith T Published Date June 2012 Volume 2012:3 Pages 25 - 39 DOI: http://dx.doi.org/10.2147/VMRR.S25021 Received: 02 March 2012 Accepted: 03 May 2012 Published: 27 June 2012 Miranda D Vieson,* Pablo Pi eyro,* Tanya LeRoith Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech University, Blacksburg, VA, USA *These authors contributed equally to this work Abstract: Numerous infectious agents are responsible for causing primary or secondary respiratory disease in dogs. These agents can cause upper or lower respiratory infections commonly observed in veterinary practices. Clinical signs might vary from mild dyspnea, sneezing, and coughing to severe pneumonia with systemic manifestations. Depending on the etiologic agent, the gross and microscopic changes observed during these infections can be rather unspecific or have highly characteristic patterns. While histopathology and cytology are not always required for diagnosis of respiratory infections, they are often useful for establishing a definitive diagnosis and identifying specific etiologic agents. Research regarding epidemiology, pathogenesis, diagnostics, and clinical manifestations related to these infectious pathogens provides valuable information that has improved treatments and management of the diseases they cause. This review discusses the epidemiology, general clinical characteristics, and pathologic lesions for some of the important viral, bacterial, fungal, and parasitic etiologies of canine respiratory disease.
A review of the pathology and treatment of canine respiratory infections  [cached]
Vieson MD,Piñeyro P,LeRoith T
Veterinary Medicine: Research and Reports , 2012,
Abstract: Miranda D Vieson,* Pablo Pi eyro,* Tanya LeRoith Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech University, Blacksburg, VA, USA *These authors contributed equally to this workAbstract: Numerous infectious agents are responsible for causing primary or secondary respiratory disease in dogs. These agents can cause upper or lower respiratory infections commonly observed in veterinary practices. Clinical signs might vary from mild dyspnea, sneezing, and coughing to severe pneumonia with systemic manifestations. Depending on the etiologic agent, the gross and microscopic changes observed during these infections can be rather unspecific or have highly characteristic patterns. While histopathology and cytology are not always required for diagnosis of respiratory infections, they are often useful for establishing a definitive diagnosis and identifying specific etiologic agents. Research regarding epidemiology, pathogenesis, diagnostics, and clinical manifestations related to these infectious pathogens provides valuable information that has improved treatments and management of the diseases they cause. This review discusses the epidemiology, general clinical characteristics, and pathologic lesions for some of the important viral, bacterial, fungal, and parasitic etiologies of canine respiratory disease.Keywords: bacterial, fungal, parasitic, pneumonia, rhinitis, tracheobronchitis, viral
Pathology of Kaposi's sarcoma-associated herpesvirus infection  [PDF]
Hideki Hasegawa,Harutaka Katano
Frontiers in Microbiology , 2011, DOI: 10.3389/fmicb.2011.00175
Abstract: Kaposi’s sarcoma-associated herpesvirus (KSHV; human herpesvirus 8) is a human herpesvirus, classified as a gamma-herpesvirus. KSHV is detected in Kaposi’s sarcoma (KS), primary effusion lymphoma (PEL), and some cases of multicentric Castleman’s disease (MCD). Similar to other herpes viruses, there are two phases of infection, latent and lytic. In KSHV-associated malignancies such as KS and PEL, KSHV latently infects almost all tumor cells. Quantitative PCR analysis revealed that each tumor cell contains one copy of KSHV in KS lesions. The oncogenesis by KSHV has remained unclear. Latency-associated nuclear antigen (LANA)-1 plays an important role in the pathogenesis of KSHV-associated malignancies through inhibition of apoptosis and maintenance of latency. Because all KSHV-infected cells express LANA-1, LANA-1 immunohistochemistry is a useful tool for diagnosis of KSHV infection. KSHV encodes some homologs of cellular proteins including cell-cycle regulators, cytokines, and chemokines, such as cyclin D, G-protein-coupled protein, interleukin-6, and macrophage inflammatory protein-1 and -2. These viral proteins mimic or disrupt host cytokine signals, resulting in microenvironments amenable to tumor growth. Lytic infection is frequently seen in MCD tissues, suggesting a different pathogenesis from KS and lymphoma.
Page 1 /100
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.