The aim of this study is to evaluate the inhibition of oxidative stress related enzymes of aqueous acetone extracts, as well as antibacterial activity from five Indigofera species well-known medicinal plant from Burkina. Also are investigated in this study the potential contribution of tannins and of flavonol in these activities Particularly, aqueous acetone extracts were investigated for their Lipoxygenase (LOX), Xanthine Oxidase (XO) and Acetylcholinesterase (AChE) inhibitions that are implied in inflammation, gout and Alzheimer’s etiology diseases. Interestingly, I. macrocalyx which had the highest flavonol content (of all) showed more inhibition against LOX and XO (51.16 and 77.33% respectively). Our study showed a significant correlation between XO inhibition and total flavonol content (R2 = 0.9052). AChE was low sensible to all extracts. In contrast, the extracts were rich in tannin compounds especially in I. tinctoria extract. And results of the in vitro antibacterial activities of these extracts against five bacteria showed that all bacteria were sensible to all extracts particularly S. typhimurium and B. cereus. Our results suggest that the five studied species prove to be good sources of inhibition of the three enzymes involved in oxidative stress and also to have some antibacterial properties. That is what probably explains their uses in folk medicine, singularly, in the treatment of gout, dysentery and anti-inflammatory diseases.