Jaenicke-Després et al. selected short DNA fragments (~50 bp) suitable for the comparison of allelic frequency from archaeological remains. DNA was extracted from cob samples ranging from 4000 to 650 years old and cloned. Reconstruction of each gene was achieved by sequencing of multiple clones. Comparison of these sequences with those from modern day maize and teosinte samples confirmed modern alleles were present in Mexican maize some 4400 years ago. The phenotypic role of the three genes investigated suggests that cob size and kernel quality were used by prehistoric farmers to select next generation plants."By 4400 years ago, early farmers had already had a substantial homogenizing effect on allelic diversity at three genes associated with maize morphology and biochemical properties of the corn cob... As more genes involved in selected features become identified in maize as well as other crops, the ability to determine nuclear gene sequences from domesticated plants recovered from archaeological excavations will make it possible to follow comprehensively the genetic consequences of domestication over time," conclude the authors."The apparent loss of genetic diversity following the introduction of high-yielding Green Revolution wheat and rice varieties in the 1960s and 1970s, and attending the rapid adoption of superior GM [genetically modified] crops today, is far from a new phenomenon," comments Nina V. Fedoroff from the Huck Institute for Life Sciences in an accompanying article.