We used morphological features and amplified fragment-length polymorphism (AFLP) markers to analyze the genetic diversity of 45 Osmanthus fragrans cultivars and found high levels of genetic diversity among the cultivars. Of the AFLP bands detected, 57.46% are polymorphic. We classified the cultivars biometrically according to their major morphological features and performed cluster analysis of the AFLP data by the unweighted pair-group method using arithmetic averages. The results of both approaches showed that O. fragrans cultivars of the Guilin region form a distinct subgroup, and flower color is aviable classification criterion. We discuss systems for O. fragrans cultivar classification.