All Title Author
Keywords Abstract


Wolbachia extensively harbored by Bemisia tabaci in China
我国烟粉虱自然种群中存在广泛的Wolbachia感染现象

Keywords: Bemisia tabaci,Wolbachia,ribosomal DNA,wsp,molecular identification
烟粉虱
,Wolbachia,rDNA,wsp,分子鉴定,烟粉虱,自然种群,存在,Wolbachia,因素,影响,灵敏度,检测方法,分子检测,显示,感染现象,发现,结果,基因,自行设计,生物型,测序鉴定,克隆,rDNA,地理种群

Full-Text   Cite this paper   Add to My Lib

Abstract:

Wolbachia are a group of maternally inherited bacteria harbored by a variety of arthropods and can ma- nipulate the reproductivity of their hosts. Eighteen populations of whiteflies were collected from Hebei, Xinjiang, Beijing, Shandong, Zhejiang, Guangxi, Hainan, Guangzhou and Fujian provinces, China. These whiteflies were molecularly identified using internal transcribed spacer 1 ITS1 rDNA sequences. They were then detected for Wolbachia infection using Wolbachia-specific primers designed based on 16S rDNA and wsp gene sequences. The results showed that almost all populations were detected positive for Wolbachia infection. Whiteflies of B / Q group mainly carried Wolbachia belonging to supergroup A, while non-B / Q whiteflies were commonly detected for Wolbachia superinfection. This study indicated that the infection rate of Wolbachia in natural populations of B. tabaci might be much higher than expected, and the threshold of detection methods may be one of the key factors influencing detection of Wolbachia infection.

Full-Text

comments powered by Disqus