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Search Results: 1 - 10 of 1130 matches for " rDNA "
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A DNA-Based Identification of Strongyloides stercor-alis Isolates from Iran
MR Nilforoushan,H Mirhendi,S Rezaie,M Rezaian
Iranian Journal of Public Health , 2007,
Abstract: Background: Strongyloides stercoralis, the etiological agent of strongyloidiasis, is one of the most common parasitic nema-tode with the unique ability to complete its life cycle and proliferate within the host. Although it is an endemic parasite in Iran, no molecular characterization is available on isolates originated from the country. Therefore, this study was con-ducted for molecular identification of human Strongyloides isolates in the three most prevalent provinces."nMethods: After microscopical examination and agar plate culture of nearly 1500 stool samples, 20 isolates of S. stercoralis filari-form larvae were recovered from (Gilan and Mazandaran in north and Khouzestan in south) of Iran. The genomic DNA was extracted from all these samples and two primer sets were selected for amplification. ITS1 region of the rDNA gene was amplified by a nested Polymerase Chain Reaction (nested-PCR). The PCR prod-ucts were sequenced and analyzed in compari-son with the sequences deposited in GenBank."nResults: DNA sequence analysis of ITS1 region showed that all the 20 isolates were S. stercoralis. There was slight varia-tion in the ITS1 region among the isolates. "nConclusion: ITS1 sequencing seems to be a valid target for molecular identification of S. stercoralis.
Chromosomal characterization of the bonytongue Arapaima gigas (Osteoglossiformes: Arapaimidae)
Marques, Debora Karla;Venere, Paulo Cesar;Galetti Junior, Pedro Manoel;
Neotropical Ichthyology , 2006, DOI: 10.1590/S1679-62252006000200007
Abstract: the mitotic chromosomes of the pirarucu arapaima gigas inhabiting the middle araguaia river and collected in the municipality of araguaiana (mt, brazil) were studied. the chromosomes were analyzed through giemsa staining, c-banding, ag-nor staining and in situ hybridization using an 18s rrna gene probe. the karyotype had 2n=56 comprising 14 biarmed and 14 uniarmed chromosome pairs in both sexes. no cytologically distinguishable sex chromosome was identified. a single nor-bearing chromosome pair was detected by ag-nor staining and confirmed by 18s rdna- fish. faint constitutive heterochromatin was c-banded in the centromeric region of some chromosomes.
Sequence analysis of the rDNA intergenic spacer of Metarhizium strains isolated in Brazil
Yanaka-Sch?fer, Fabiana Y.;Dall'Onder, Leonara P.;Panichi, Mariana C.;Mendes, Roberta G.;Fagundes, Nelson J.R.;Bandinelli, Josiane B.;Bogo, Maurício R.;
Genetics and Molecular Biology , 2008, DOI: 10.1590/S1415-47572008000100020
Abstract: to assess the extent of genetic variability of rdna intergenic spacer (igs) in metarhizium sp., 34 strains (27 isolated in brazil) were sequenced and analyzed together with an additional 20 metarhizium anisopliae var. anisopliae sequences retrieved from genbank. overall, the global nucleotide diversity for the region under study was of 0.090, while for the brazilian isolates it was only 0.016. phylogenetic analyses showed four well-supported groups (a, b, c, and d), one of which (d) has not been previously identified. all but one of the brazilian strains cluster in this novel d phylogroup, suggesting that the genetic variation found in brazil is a subset of the worldwide m. anisopiliae var. anisopliae variation.
Description of karyotype in Hypostomus regani (Ihering, 1905) (Teleostei, Loricariidae) from the Piumhi river in Brazil with comments on karyotype variation found in Hypostomus
Ernani de Oliveira Mendes-Neto,Marcelo Ricardo Vicari,Roberto Artoni,Orlando Moreira-Filho
Comparative Cytogenetics , 2011, DOI: 10.3897/compcytogen.v5i2.964
Abstract: The paper represents a comparative cytogenetic analysis of three populations of Hypostomus regani in Brazil. Two populations belong to the Upper Paraná River Basin and the third one, the karyotype of which is described for the first time, was probably introduced into the S o Francisco River Basin through transposition from the Piumhi River. Karyotype features of populations of H. regani from the Piracicaba and Tietê River Basins are also discussed. The occurrence of H. regani in the S o Francisco River Basin is reported for the first time here. The study also revealed distinct differences in the location of the Ag-NORs between the analyzed populations that enable individuals from the Piumhi River, Mogi-Gua u River and Tietê River to be distinguished from one another. Thus, the data obtained indicate the possibility of geographic variation fixing different karyotypes even in the same basin of origin.
麂属rDNA限制性位点多态性及其系统进化
兰宏
科学通报 , 1993,
Abstract: 核糖体RNA基因家族(rRNAgene或rDNA)是一类中等重复的DNA序列,每个重复单元由非转录间隔区(nontranscribedspacer,NTS)、转录间隔区(internaltranscri-bedspacer,ITS)和三种rRNA(18SRNA,5.8SRNA,28SRNA)基因编码区组成.由于rDNA遵循协同进化(concertedevolution)模式,种内个体间重复单元的序列差异不
Genetic Relatedness of Diplostomum Species (Digenea: Diplostomidae) Infesting Nile Tilapia (Oreochromis Niloticus L.) in Western Kenya  [PDF]
Violet M. Ndeda, Dickson O. Owiti, Ben O. Aketch, David M. Onyango
Open Journal of Applied Sciences (OJAppS) , 2013, DOI: 10.4236/ojapps.2013.38055
Abstract: Diplostomum species metacercariae are trematode parasites that pose serious economic threats to aquaculture practice globally. Identification of Diplostomum at metacercariae stage has remained equivocal, hence lack of elucidation of the actual role of these species in fish population. 21 Diplostomoid metacercariae obtained from eyes of Nile tilapia were characterized using 18S and ITS rDNA (ITS1-5.8S-ITS2) genes. Phylogenetic analyses of ITS rDNA gene dataset in the metacercariae revealed close relationship to Diplostomum mashonense and D. baeri. Molecular identification using 18S rDNA sequences revealed close relationship to D. compactum, D. phoxini and D. spathaceum. Overall, genetic analyses in this study depicted a significant unrecognized genetic diversity among Diplostomum species. Successful differentiation of Diplostomum genera in this study using ribosomal markers suggested that 18S and ITS rDNA genes are effective genetic markers for inter-species phylogenetic analysis and should be employed in future for identification of diplostomoidea.
Genetic Diversity of Marine and Fresh Water Cyanobacteria from the Gujarat State of India  [PDF]
Nilkanth Faldu, Shivani Patel, Nutan Prakash Vishwakarma, Anil Kumar Singh, Khushbu Patel, Neepa Pandhi
Advances in Bioscience and Biotechnology (ABB) , 2014, DOI: 10.4236/abb.2014.514121
Abstract: Cyanobacteria from habitats within Gujarat have been poorly studied with regard to their diversity. In the present investigation eight morphologically distinct cyanobacterial isolates were obtained and characterized from the fresh water and marine habitats. Identification was performed based on morphological features and on 16S rDNA sequences analysis. A phylogenetic tree based on 16S rDNA sequence of cyanobacterial isolates was prepared. Phylogenetic analysis clustered the eight morphologically distinct isolates into two distinct groups thus highlighting the importance of both morphological and genetic methods in studying cyanobacterial diversity.
一株产纤维素酶甲醇耐受细菌的分离与鉴定
Separation and Identification of a Bacterum Which Can Produce Cellulase and Tolerate Methanol
 [PDF]

嵇朝球, 祁鹏飞, 裴坤, 马中良
Hans Journal of Chemical Engineering and Technology (HJCET) , 2016, DOI: 10.12677/HJCET.2016.66023
Abstract:
当今时代,生物能源已经成为一种绿色经济的能源。为了更有效地利用纤维素,在这项研究中,自江苏盐城工业园区采集的土样中分离到一株既可耐受甲醇,又可产生高活性纤维素酶的细菌。通过16S rDNA序列分析,构建系统发育树,该菌株与巨大芽孢杆菌(B. megaterium)相似性为99.7%。甲醇耐受实验表明,菌株具有较高的甲醇耐受,甚至能在11.85%甲醇的M9培养基中生长。
Bioenergy is a green and economic energy in modern times. To have Celluose used more efficiently, in this study, from soil samples in Yancheng Industrial Park of Jiangsu, we isolated a bacterium that cannot only grow in the presence of methanol, but also produce higher activity cellulase Provice. By sequencing analysis of 16S rDNA, the phylogenetic tree was constructed, and the similarity between the strain and B. megaterium was 99.7%. The testing of tolerance in methanol showed that the strains had higher tolerance of methanol and could even grow in the M9 medium with 11.85% methanol.
新科Rhizoplacopsidaceae中的新属及新种Rhizoplacopsis weichingii
周启明,魏江春
菌物学报 , 2006,
Abstract: 本文描述了位于新科盾叶科 Rhizoplacopsidaceae(Umbilicariales,Lecanoromycetes,Ascomycota)中的新属盾叶属 Rhizoplacopsis 和新种蔚青盾叶 Rhizoplacopsis weichingii。蔚青盾叶取名于著名中国真菌学家王云章教授之字“蔚青”,作为庆祝教授百岁生日之纪念。该新种在外形上与 Rhizoplaca 属地衣极为相似,但位于地衣体上的子囊盘却为网衣型。此外,它的子囊顶器结构非常接近于 Umbilicaria-type。基于分子数据,对 Rhizoplacopsis weichingii 及其它相关地衣进行的系统发育分析支持成立新属盾叶属 vRhizoplacopsis 和新科盾叶科 Rhizoplacopsidaceae。
Phylogeny and ecology of the ubiquitous saprobe Cladosporium sphaerospermum, with descriptions of seven new species from hypersaline environments
P. Zalar,G.S. de Hoog,H.-J. Schroers,P.W. Crous
Studies in Mycology , 2007,
Abstract: Saprobic Cladosporium isolates morphologically similar to C. sphaerospermum are phylogenetically analysed on the basis of DNA sequences of the ribosomal RNA gene cluster, including the internal transcribed spacer regions ITS1 and ITS2, the 5.8S rDNA (ITS) and the small subunit (SSU) rDNA as well as -tubulin and actin gene introns and exons. Most of the C. sphaerospermum-like species show halotolerance as a recurrent feature. Cladosporium sphaerospermum, which is characterised by almost globose conidia, is redefined on the basis of its ex-neotype culture. Cladosporium dominicanum, C. psychrotolerans, C. velox, C. spinulosum and C. halotolerans, all with globoid conidia, are newly described on the basis of phylogenetic analyses and cryptic morphological and physiological characters. Cladosporium halotolerans was isolated from hypersaline water and bathrooms and detected once on dolphin skin. Cladosporium dominicanum and C. velox were isolated from plant material and hypersaline water. Cladosporium psychrotolerans, which grows well at 4 °C but not at 30 °C, and C. spinulosum, having conspicuously ornamented conidia with long digitate projections, are currently only known from hypersaline water. We also newly describe C. salinae from hypersaline water and C. fusiforme from hypersaline water and animal feed. Both species have ovoid to ellipsoid conidia and are therefore reminiscent of C. herbarum. Cladosporium langeronii (= Hormodendrum langeronii) previously described as a pathogen on human skin, is halotolerant but has not yet been recorded from hypersaline environments.
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