In this paper, the spatio\|temporal distribution of arbuscular mycorrhiza (AM) fungi and glomalin in rhizosphere of Artemisia ordosica and its relationship with soil factors were investigated in sand soil north to Yulin city, Shanxi, China. In April, July and October, 2007, soil samples around rhisophere of A.ordosica were collected. Each time, the soil was divided into five layers on the basis of depth from top to bottom: 0-10 cm, 10-20 cm, 20-30 cm, 30-40 cm, 40-50 cm, respectively. The results showed that the total colonization rate (89.54%), vesicular colonization rate (26.24%) and arbuscule colonization rate (21.08%) of AM fungi were very high around rhisophere of A.ordosica, and spore number was 2.91-6.17 per gram soil, indicating that AM fungi established well symbiosis with rhisophere of A. ordosica. Twenty\|one species of AM fungi in four genera were detected in soil samples, the predominant genera and predominant species was Glomus and G. geosporum, respectively. Distribution of AM fungi and glomalin was remarkably heterogeneous and closely related with soil factors. Hypha colonization increased gradually with season. Vesicular colonization rate and arbuscule colonization rate were at their lowest level in summer and higher in spring and autumn, seasonal variation of spore density was in a reverse pattern. Total extractable glomalin (TEG) was negatively correlated with soil depth, the highest value was in surface (0-20 cm) layer. Easy\|extracted glomalin (EEG) content showed a fluctuation with soil depth. The TEG content was high in spring and decreased in summer and autumn. TEG and EEG were remarkably correlated with soil nutrition, soil enzyme activity and spore density of AM fungi, in future research, they can be used as parameters to monitor the development of AM fungi community, organic carbon dynamic and nutrition cycle in sand soil.