All Title Author
Keywords Abstract

Publish in OALib Journal
ISSN: 2333-9721
APC: Only $99

ViewsDownloads

Relative Articles

Intensive Farmaco- Surveillance of Interferon Alpha 2b Recombinant in Multiple Sclerosis. Farmacovigilancia intensiva al interferón alfa 2b recombinante en la esclerosis múltiple.

Efecto antifibrótico del interefrón alfa 2b recombinante en la cirrosis hepática por virus B o C Antifibrotic effect of recombinant interferon alfa 2b in liver cirrhosis caused by virus B or C

Experiencia con las reacciones adversas asociadas con el interferón alfa 2b recombinante en hematología Experience with the adverse reactions associated with recombinant alpha 2b interferon in hematology

Seguridad de la terapia de interferón alfa 2b recombinante más ribavirina en la hepatitis crónica C Safety of recombinant interferon alpha 2b plus ribavirin in chronic hepatitis C

Polineuropatia desmielinizante inflamatória cr?nica pós-tratamento com interferon peguilado alfa 2b em um paciente co-infectado HIV/HCV: relato de caso

Interferón alfa 2b recombinante más ribavirina en el tratamiento de la hepatitis crónica C Recombinant Interferon alfa-2b plus Ribavirin in treatment of chronic hepatitis C

Interferon alfa a opryszczkowate zapalenie skóry

Pegylated interferon alfa and ribavirin for children with chronic hepatitis C

Treatment with pegylated interferon alpha 2b and ribavirin in patients unresponsive to previous treatments with standard interferon as monotherapy or combined with ribavirin Tratamiento con PEG interferón alfa 2b y ribavirina en enfermos previamente tratados con interferón estándar en monoterapia o combinado con ribavirina

Mania associated with interferon α2b treatment

More...

A simple method for quantification of interferon- &alfa;2b through surface plasmon resonance technique

Full-Text   Cite this paper   Add to My Lib

Abstract:

A rapid and efficient immunoassay method for quantification of interferon-2b using surface plasmon resonance was developed with BIAcore 3000 as a sensor. Two different levels of anti-interferon monoclonal antibody were immobilized onto a CM5 chip using an amine coupling method. Similar binding ratio was observed for both the ligand densities. There was no steric hindrance and loss of antibody activity even at higher ligand density (> 22,000 RU). The sensitivity of the assay was increased up to 45% with the increment in ligand density from 15,400 to 22,360 RU. The binding between interferon-2b and anti-interferon monoclonal antibody was predominantly controlled by mass transfer rate and the relationship was found linear, ranged from 5 to 400 ng/mL. Total cycle time per analysis was less than 8 min and required only 5 L of sample injection.

Full-Text

comments powered by Disqus