objectives: to determine the prevalence of chlamydia trachomatis infection in infertile couples. to evaluate the efficiency of the chlamydia trachomatis screening programs. to establish the need of implementing the routine early diagnosis and opportune treatment of the infection. methods: prevalence of chlamydia trachomatis infection was determined in 4 019 patients, 1 991 women and 2 012 men in reproductive agesbetween 1999 and 2008. anti-chlamydia and c.trachomatis antobodies (igg, iga and igm) were detected by seroelisa (savyon diagnostics ltd.) from 1999 to 2005; and by the immunocomb ii kit (orgenics) from 2006 to 2008. setting: fertility clinic unifertes in caracas, venezuela. results: a prevalence of 25,40 ± 6,26 % was found in women and a prevalence of 31,12 ± 2,88 % was found in men. conclusions: prevalence of the c. trachomatis infection in infertile couples is high and has not decreased over the last 10 years. implementation of routine screening programs for c. trachomatis detection is recommended, including its assessment in annual gynecological controls, as well as in urologic evaluations in young men, in order to prevent the infection from being chronic.