aerosol kits are used in treatments of respiratory illnesses and require at least an intermediate level of disinfection for their reuse. the objectives of the study were to verify possible contamination of kits after they were processed as well as to identify the microorganisms that were isolated in them. this transversal experimental study was conducted in the pediatric unit of a hospital in goiania, state of goiás. samples were collected from three different parts (masks, cups and hoses) of 15 previously disinfected kits and placed in different culture media, and the microorganisms that were isolated were identified through biochemical tests. of the kits that were analyzed, 13 cups, 9 hoses and 13 masks were found to be contaminated. a total of 101 cfu were isolated 39 from the cups, 20 from the hoses, and 42 the masks. among the pathogens found were coagulase-positive staphylococci, coagulase-negative staphylococci, gram negative fermenter rods, gram negative nonfermenter rods, micrococcus and yeast. microbial presence indicates probable inefficiencies in processing these items.