it is known that micronutrients and growth regulators are important metabolism activators, which can bring benefits to germination and seed vigor when incorporated into the treatment and so increase the developmental potential of the plants in the field. the objective of this study was to evaluate the effect of applying micronutrients and growth regulators in germination, vigor, on the activity of some enzymes and on the total protein contents in lettuce seeds during storage. the seeds were treated with the chemicals starter？, cellerate？ and stimulate？ at the dosages corresponding to 0%, 50%, 100%, 150% and 200% of the dose recommended by the manufacturer, using the film-coating technique. the evaluations were performed at 0, 6 and 12 months of storage on the following parameters: germination percentage, emergence percentage and speed index, activity of enzymes endo-β-mannanase and esterase, total protein content and health. it was concluded that growth regulators promoted an increase in the emergence speed index of the lettuce seedlings when applied at the dose recommended and at pre-sowing; the coating of the lettuce seeds with the double the recommended dose of the chemicals based on micronutrients and growth regulators, reduced their quality; the activity of the esterase enzyme increases with the storage of lettuce seeds, pointing to an increase in the decaying process; the coating with both micronutrients and growth regulators and storage interfere in the activity of the endo-β-mannanase enzyme in lettuce and seeds.